血清抗环瓜氨酸肽抗体类风湿因子检测对类风湿关节炎的早期诊断价值研究

目的：探讨抗环瓜氨酸肽抗体（抗CCP抗体）及类风湿因子（RF）检测在类风湿关节炎（RA）早期诊断中的研究价值。方法采用酶联免疫吸附测定法（ELISA）和免疫散射比浊法检测86例类风湿关节炎患者（RA组）和62例非风湿性自身免疫疾病患者（非RA组）的血清抗环瓜氨酸肽抗体水平及类风湿因子的水平，分析抗CCP抗体及类风湿因子对类风湿关节炎的诊断价值。结果 RA组的抗CCP抗体、RF及联合检测的阳性检出率均高于非RA组患者，且RA组联合检测的阳性检出率显著高于单一指标的检出率（P＜0．05）；抗CCP抗体的特异度及阳性检测值显著高于RF（P＜0．05），联合检测的灵敏度及阴性检测值显著高于抗CCP抗体及RF（P＜0．05）。结论抗CCP抗体及RF是RA早期诊断的重要指标，二者联合检测可以有效提高RA临床诊断的准确性，为与其他结蹄组织疾病的区别提供依据。

The value of serum anti-CCP antibody and rheumatoid factors in rheumatoid arthritis early diagnosis

Objective To explore the value of serum anti‐cyclin citrullinated peptide(anti‐CCP)antibody and rheumatoid factore (RF)in rheumatoid arthritis early diagnosis .Methods Using enzyme‐linked immunosorbent assay(ELISA)and immunity transmis‐sion turbidimetry(ITA)methods to detect the anti‐CCP antibody and RF serum level in 86 cases RA patients(RA group)and 62 case non‐RA autoimmune disease patients(non‐RA group) ,then analyzed the diagnosis value of these two factors .Results The positive rates of anti‐CCP antibody ,RF and combined detection in RA group were significantly higher than non‐RA group ,meanwhile ,the rate of combined detection was higher than the others in RA group (P<0 .05);The specificity and positive values of anti‐CCP anti‐body were obviously higher than RF(P<0 .05);The sensitivity and negative value of combined detection were better than anti‐CCP antibody and RF(P<0 .05) .Conclusion Both anti‐CCP antibody and RF are the important creterias in rheumatoid dignosis ,com‐bined these two factors can significantly improve the accuracy of RA clinical diagnosis and contribute to distinguish from orher con‐nective tissue disease .