福辛普利对糖尿病大鼠心功能的保护

目的:观察福辛普利对糖尿病大鼠心功能、心肌局部血管紧张素Ⅱ(AngⅡ)浓度及转化生长因子(TGF-β1)表达的影响。探讨福辛普利通过AngⅡ和TGF-β1保护心功能的机制。方法:30只SD大鼠,随机取20只大鼠建立糖尿病模型后,再随机分为福辛普利治疗组(干预组)及糖尿病未治疗组(未治疗组),每组10只,另10只作为正常对照组。治疗组以福辛普利15 mg/(kg.d)水溶液灌胃,对照组和未治疗组每天以同等体积清水灌胃。9周后,用超声心动图观察心功能,放射免疫法测定心肌局部AngⅡ的水平,用免疫组织化学法检测心肌细胞中TGF-β1的表达含量。结果:糖尿病未治疗组心肌局部AngⅡ含量显著高于正常对照组(5 367.43±498.28和4 340.67±348.37,P<0.01),福辛普利干预组AngⅡ含量显著降低(4 501.50±129.33,P<0.01)。未治疗组心肌局部的TGF-β1表达显著高于对照组(0.266 5±0.049 3和0.135 2±0.050 7,P<0.01);福辛普利干预后TGF-β1表达则明显降低(0.140 9±0.039 6,P<0.01)。未治疗组心功能明显低于对照组(P<0.01);福辛普利干预组心功能显著改善(P<0.01)。相关分析表明:未治疗组心肌局部AngⅡ含量与心肌TGF-β1表达明显正相关(r=0.802,P<0.05);而AngⅡ的含量和TGF-β1的表达分别与心功能明显负相关(r=-0.832P<0.05,r=-0.776,P<0.05)。结论:福辛普利能通过降低心肌局部AngⅡ含量而减少心肌TGF-β1表达,减轻心功能的恶化。

Protection of Cardiac Function of Diabetic Rats by Fosinopril

Objective: To observe the effect of fosinopril on the change of cardiac function,the content of AngⅡ in the rat homogenate of heart muscle and the expression of TGF-β1 in myocardium in diabetic rats.Methods: A total of 30 SD rats were enrolled in the study,ten of them were set as normal controls,others were injected with STZ to construct diabetic models to study the effect of fosinopril.Doppler echocardiography was adopted to determine the cardiac fuction,and the content of AngⅡ in the myocardium was assayed by radio-immunoassay.By the method of immunochemistry,the expression of TGF-β1 in the ventricular cells was detected as the index to evaluate the degree of fibrosis.Results: When compared with those of untreated model group,cardiac function(E/A) was significantly improved(P<0.01),and the expression of TGF-β1 decreased(P<0.01) in the fosinopril treated group.These two indices were significantly correlated with the content of AngⅡ of heart(r=-0.832,P<0.05;r=0.802,P<0.05).Conclusion: Fosinopril can inhibit the expression of TGF-β1 and elevate cardiac function through deceasing the content of AngⅡ of myocardium.