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| 转铁蛋白与Bel-7402肝癌细胞耐药性关系的研究 | |
| 引用本文： | 谭文彬,周广起,王霄,路海,亚白柳,刘永春,李士根,郭永和. 转铁蛋白与Bel-7402肝癌细胞耐药性关系的研究[J]. 中国热带医学, 2012, 12(3): 288-289,317 |
| 作者姓名： | 谭文彬 周广起 王霄 路海 亚白柳 刘永春 李士根 郭永和 |
| 作者单位： | 1. 济宁医学院,山东济宁,272067 2. 济宁医学院附属医院,山东济宁,272029 |
| 基金项目： | 山东省教育厅课题,济宁市优秀中青年科研创新资金计划项目,山东省自然科学基金 |
| 摘 要： | 目的 研究转铁蛋白与Bel-7402肝癌细胞耐药性的关系.方法 根据已知转铁蛋白序列设计引物扩增基因片段,荧光定量PCR检测其在敏感Bel-7402细胞株、阿霉素抗性Bel-7402细胞株之间的表达水平差异.结果 成功扩增Bel-7402肝癌细胞转铁蛋白基因片段,荧光定量PCR表明转铁蛋白基因在阿霉素抗性Bel-7402细胞株的表达量是敏感Bel-7402细胞株4.83倍.结论 转铁蛋白基因对肝癌细胞的耐药发生发展存在一定的作用,可为研究肝癌耐药性的备选基因,以及为肝癌耐药性检测提供新的靶标. |
| 关 键 词： | 转铁蛋白 BEL-7402 荧光定量PCR 肝癌细胞 耐药性 |
The relationship between transferrin and drug resistance of BEL-7402 hepatocellular carcinoma cell line |
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| Affiliation: | TAN Wen-bin,ZHOU Guang-qi,WANG Xiao,et al.(1.Jining Medical College,Jining 272067,Shandong,P.R.China) |
| Abstract: | Objective To study the relationship between transferrin and drug resistance of BEL-7402 hepatocellular carcinoma cell line.Methods The primers for amplification of transferrin gene fragment on the basis of known gene sequence were designed,fluorescence quantitative PCR was used for detection of expression level difference between sensitive BEL-7402 hepatocellular carcinoma cell line and adriamycin resistant BEL-7402 hepatocellular carcinoma cell line.Results The transferrin gene fragment was cloned successfully and FQ-PCR proved the transferrin gene expression level of adriamycin resistant BEL-7402 hepatocellular carcinoma cell line was 4.83 times higher than that of sensitive BEL-7402 hepatocellular carcinoma cell line.Conclusion The transferrin gene presents a certain role in drug resistance development mechanism of hepatocellular carcinoma cell line,and it can be used as a new resistance gene and drug resistance detection target. |
| Keywords: | Transferrin BEL-7402 Fluorescence quantitative PCR Hepatocellular carcinoma cell Drug resistance |
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