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| 片姜黄HPLC指纹图谱研究 | |
| 引用本文： | 户光,陈两绵,宋蓓,朱晶晶,刘守金.片姜黄HPLC指纹图谱研究[J].中国实验方剂学杂志,2012,18(8):106-110. |
| 作者姓名： | 户光 陈两绵 宋蓓 朱晶晶 刘守金 |
| 作者单位： | 1. 安徽中医学院,合肥230038;中国中医科学院中药研究所,北京100700 2. 中国中医科学院中药研究所,北京100700;中药质量控制技术国家工程实验室,北京100700 3. 安徽中医学院,合肥,230038 |
| 基金项目： | 国家青年科学基金项目(30901961);中国中医科学院中药研究所基本科研业务费自主选题项目(ZZ20090215) |
| 摘 要： | 目的:建立不同产地片姜黄药材的指纹图谱.方法:采用HPLC,建立了16批不同产地的片姜黄药材的指纹图谱.色谱柱Kromasil 100-5C18(4.6 mm×250 mm,5μm),流动相乙腈-水溶液梯度洗脱,柱温35℃,流速0.8 mL· min-1,检测波长244 nm,提取41个色谱峰作为化学性状,分别采用相似度评价,聚类分析和主成分分析等方法,对所收集的16批样品进行系统的比较与归类.结果:建立片姜黄专属性的指纹图谱,确定了14个共有峰,将不同产地的样品分为三大类.结论:该方法重复性好,简便可靠,可用于片姜黄药材的鉴别,可以为片姜黄的质量控制和临床使用提供依据. |
| 关 键 词： | 片姜黄 指纹图谱 高效液相 聚类分析 主成分分析 |
| 收稿时间： | 2011/12/23 0:00:00 |
Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum |
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| HU Guang,CHEN Liang-mian,SONG Bei,ZHU Jing-jing and LIU Shou-jin.Study on the HPLC Fingerprint of Rhizoma wenyujin Concisum[J].China Journal of Experimental Traditional Medical Formulae,2012,18(8):106-110. | |
| Authors: | HU Guang CHEN Liang-mian SONG Bei ZHU Jing-jing and LIU Shou-jin |
| Institution: | Anhui University of Traditional Chinese Medicine, Hefei 230038, China; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Anhui University of Traditional Chinese Medicine, Hefei 230038, China |
| Abstract: | Objective:To find a quality evaluation method of Concisum Rhizoma Wenyujin.Method: HPLC fingerprint profiles of sixteen samples were established.Column Kromasil 100-5C18(4.6 mm×250 mm,5 μm) was used.The mobile phase was acetonitrile and water,and the flow rate was 0.8 mL·min-1.A matrix including 41 variations(characteristic peaks area) and 16 cases(samples) was constructed for similarity evaluation,cluster analysis and principle component analysis.Result: The results showed that the collected samples had a good similarity.A specificity fingerprint chromatogram was produced and fourteen characteristic peaks were designated.Sixteen samples were divided into three groups.Conclusion: It was a reliable and available method of quality control of Rhizoma wenyujin concisum and it was necessary for the safe and practicable use of this herbal medicine. |
| Keywords: | Rhizoma Wenyujin Concisum HPLC fingerprint Similarity evaluation Cluster analysis Principal Component Analysis |
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