蝴蝶草提取物体内抗肿瘤作用的研究

目的:观察壮药蝴蝶草提取物体内抗肿瘤作用.方法:取生长良好的小鼠移植肿瘤S180,H22细胞,在小鼠前肢腋窝皮下接种0.2 mL瘤细胞悬液,接种24 h后,随机分成5组,即蝴蝶草提取物低、中、高剂量组,荷瘤对照组,阳性对照组,每组10只,雌雄各半,蝴蝶草提取物低、中、高剂量组分别为3,6,12 g·kg-1,ig每天1次,连续给药10d,观察壮药蝴蝶草提取物对S180肉瘤小鼠和H22肝癌小鼠的抑瘤率,对荷瘤小鼠的免疫功能和造血系统的影响,以及对荷瘤小鼠生命延长率的影响.结果:与荷瘤对照组比,壮药蝴蝶草提取物对小鼠移植肿瘤S180,H22具有明显的抑制作用(P＜0.01或P＜0.05),其中高剂量的抑瘤率分别为54.42％,60.77％；能显著提高S180肉瘤小鼠和H22肝癌小鼠的生命延长率(P＜0.01或P＜0.05),与哪一组比较？其中高剂量的延长率分别为82.13％,83.10％.对荷瘤小鼠的免疫功能(脾脏指数和胸腺指数)和造血系统无明显影响.结论:壮药蝴蝶草提取物对荷瘤S180和H22小鼠肿瘤增殖有较好的抑制作用,能明显延长荷瘤小鼠的生存时间.

Study on Antitumor Effect of the Extract from Christia vespertilionis in vivo

Objective: To observe the antitumor effects of extract from Christia vespertilionis in vivo. Method: The mice S180-mice model and H22-mice model were established by inoculation into subaxillary with 0.2 mL tumor cell suspension. After 24 h of inoculation, mice were randomly divided into five groups, extract from C. vespertilionis low-dose, middle-dose and high-dose group, tumor-bearing control group, positive control group. The extract from C. vespertilionis at dose of 3, 6, 12 g·kg^-1, was ig administrated,for 10 days. The tumor-inhibition rate of extract from C. vespertilionis were tested with S180-mice model and H22-mice model in vivo, the immune function and hematopoietic system of tumor-bearing mice were observed, and the S180-mice model and H22-mice model was used to test the survival rate. Result: Compare to the tumor-bearing control group, the extract from C.vespertilionis could significantly inhibit the growth of S180 tumor and H22 tumor cells(P<0.01 or P<0.05), and the inhibition rate at high-dose group nwas 54.42%, 60.77%, respectively. The extract could also greatly prolong life of the mice with S180 and H22 ascitic tumor, and life span at high-dose was 82.13%, 83.10%,respectively. No significant changes in immune function and hematopoietic system of tumor-bearing mice were found. Conclusion: The extract from C.vespertilionis is able to significantly inhibit tumor growth and prolong the survival period of the tumor-bearing mice.