肝细胞生长因子对人Tenon's囊成纤维细胞低密度脂蛋白受体表达的影响

目的:探讨人Tenon's囊成纤维细胞(HTFs)在肝细胞生长因子(HGF)刺激下,其低密度脂蛋白受体(LDLr)表达的动态变化。方法:分别以0、10、20、40、80和160μg/L的HGF刺激人Tenon's囊成纤维细胞12、24和48 h;应用MTT法检测HTFs的增殖率;real-time PCR定量分析不同增殖状态的HTFs上LDLr mRNA的表达水平;Western blot法检测不同增殖状态HTFs上LDLr蛋白表达水平的变化。结果:HTFs上LDLr mRNA及蛋白的表达水平均与HTFs的增殖率呈正相关,HGF以时间和浓度依赖的方式促进HTFs的增殖,同时以时间和浓度依赖的方式促进LDLr的表达(P0.05)。结论:在HGF刺激下,增殖活跃的HTFs上LDLr呈高表达状态,提示增殖异常活跃的HTFs上高表达的LDLr,可能成为一种新型的抗代谢药物作用靶点,尤其是在抗青光眼术后滤过泡瘢痕化的研究中可能得到广泛应用。

Expression of low-density lipoprotein receptor after exposure to hepatocyte growth factor in human Tenon's capsule fibroblasts

AIM: To study the dynamic alteration of low-density lipoprotein receptor (LDLr) expression after exposure to hepatocyte growth factor (HGF) in human Tenon's capsule fibroblasts (HTFs).METHODS: HTFs were stimulated with HGF at different concentrations (0, 10, 20, 40, 80 and 160 μg/L) for 12, 24, and 48 h. The viability of HTFs was analyzed by MTT assay. The expression of LDLr at mRNA and protein levels were analyzed by real-time PCR and Western blot.RESULTS: The expression of LDLr at mRNA and protein levels was positively correlated with the viability of HTFs. HGF promoted the viability of HTFs in a time-and concentration-dependent manner. At the same time, HGF promoted the expression of LDLr in the same manner.CONCLUSION: Exposure of HTFs to HGF induces LDLr expression at high level, suggesting that over-expression of LDLr on the HTFs may be a target receptor for controlled drug delivery, particularly in anti-scarring therapy after glaucoma filtration surgery.