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| Apaf-1在甲状腺乳头状癌中的表达及意义 | |
| 引用本文： | 李益波,陈冠桦,戴岳楚,伍慧慧,麻怀露,俞盛健,梁勇. Apaf-1在甲状腺乳头状癌中的表达及意义[J]. 中国病理生理杂志, 2019, 35(1): 55-61. DOI: 10.3969/j.issn.1000-4718.2019.01.009 |
| 作者姓名： | 李益波 陈冠桦 戴岳楚 伍慧慧 麻怀露 俞盛健 梁勇 |
| 作者单位： | 1. 河北北方学院, 河北 张家口 075000; 2. 台州学院, 浙江 台州 318000; 3. 温州医科大学第一临床学院, 浙江 温州 325000; 4. 台州学院附属医院, 浙江 台州 318000 |
| 基金项目： | 浙江省自然科学基金资助项目（No.LY14H160036）；台州市科技计划项目（No.1501KY31） |
| 摘 要： | 目的:探讨凋亡蛋白酶活化因子1(Apaf-1)在甲状腺乳头状癌(PTC)中的m RNA与蛋白表达及其与细胞增殖的关系。方法:分别采用real-time PCR、Western blot及免疫组化法检测并比较甲状腺乳头状癌和癌旁组织中Apaf-1的m RNA及蛋白表达情况,并分析其表达水平与PTC临床病理学特征的关系;通过下调CGTHW-3细胞中Apaf-1表达量,验证Apaf-1对细胞增殖的影响。结果:在PTC组织中,Apaf-1的m RNA和蛋白表达量均显著低于癌旁组织(P <0. 05);下调Apaf-1表达增强了CGTHW-3细胞的增殖活性(P <0. 05)。结论:Apaf-1在PTC中低表达,抑制其表达可增强CGTHW-3细胞的增殖能力。Apaf-1在甲状腺乳头状癌中可能发挥抑癌作用。 |
| 关 键 词： | 凋亡蛋白酶活化因子1 甲状腺乳头状癌 细胞增殖 |
| 收稿时间： | 2018-04-03 |
The expression and significance of Apaf-1 in papillary thyroid carcinoma |
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| LI Yi-bo,CHEN Guan-hua,DAI Yue-chu,WU Hui-hui,MA Huai-lu,YU Sheng-jian,LIANG Yong. The expression and significance of Apaf-1 in papillary thyroid carcinoma[J]. Chinese Journal of Pathophysiology, 2019, 35(1): 55-61. DOI: 10.3969/j.issn.1000-4718.2019.01.009 | |
| Authors: | LI Yi-bo CHEN Guan-hua DAI Yue-chu WU Hui-hui MA Huai-lu YU Sheng-jian LIANG Yong |
| Affiliation: | 1. Hebei North University, Zhangjiakou 075000, China; 2. Taizhou University, Taizhou 318000, China; 3. The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China; 4. The Affiliated Hospital, Taizhou University, Taizhou 318000, China |
| Abstract: | AIM: To investigate the mRNA and protein expression of apoptotic protease-activating factor 1 (Apaf-1) in papillary thyroid carcinoma (PTC) and its relationship with cell proliferation.METHODS: The methods of real-time PCR, Western blot and immunohistochemistry were used to detect the mRNA and protein expression of Apaf-1 in PTC and tumor-adjacent tissues. The relationship between Apaf-1 expression and clinicopathological characteristics was analyzed. The effect of Apaf-1 on cell proliferation was verified by down-regulating the expression of Apaf-1 in CGTHW-3 cells.RESULTS: The mRNA and protein expression levels of Apaf-1 in the PTC tissue were significantly lower than those in the tumor-adjacent tissue (P<0.05). Down-regulation of Apaf-1 expression enhanced the proliferation of CGTHW-3 cells (P<0.05).CONCLUSION: The expression of Apaf-1 is low in PTC, and inhibition of its expression enhances the proliferation of CGTHW-3 cells. Apaf-1 may play a tumor suppressor role in PTC. |
| Keywords: | Apoptotic protease-activating factor 1 Papillary thyroid carcinoma Cell proliferation |
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