自噬对HIV-1 gp120V3环介导的海马神经元L型钙离子通道电流的影响

 目的:观察哺乳动物雷帕雷素靶蛋白（mTOR）依赖性自噬信号转导通路对HIV-1 gp120V3环介导的海马神经元L-型钙离子通道电流的影响。方法：取出生1 d内的乳鼠海马神经元原代培养7 d后用于实验。实验分为2个平行实验组,即空白对照组、gp120V3组、自噬阻断剂3-甲基腺嘌呤（3-MA）组和gp120V3+3-MA组;空白对照组、gp120V3组、自噬激动剂雷帕雷素（rapamycin)组和gp120V3+rapamycin组。以膜片钳技术记录海马神经元的L型钙离子通道电流。结果：gp120V3组及3-MA组与空白对照组比较,L型钙离子通道电流密度增大（P<0.05）;gp120V3+3-MA组与gp120V3组比较,L-型钙离子通道电流密度增大（P<0.05）;rapamycin组与空白对照组比较,L型钙离子通道电流密度减小（P<0.05）;gp120V3+rapamycin组与gp120V3组比较,L型钙离子通道电流密度减小（P<0.05）。结论：从电生理角度证明,自噬可能参与了HIV-1gp120V3环介导的海马神经元L型钙离子通道电流的改变。

Effect of autophagy on L-type calcium channel current induced by gp120V3 in hippocampus neurons

AIM: To observe the influence of autophagy on L-type calcium channel current induced by gp120V3 loop in hippocampal neurons.METHODS: Hippocampal neurons were exteriorized from the newborn rats within 1 d and primarily cultured for 7 d. The neurons were divided into 2 parallel groups, namely control group, gp120V3 group, autophagy inhibitor 3-MA group, gp120V3 + 3-MA group; control group, gp120V3 group, autophagy activator rapamycin group, gp120V3 + rapamycin group. Whole-cell pathch-clamp was used to record L-type calcium channel current. RESULTS: Compared with control group, the density of L-type calcium channel current increased in gp120V3 group and 3-MA group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + 3-MA group(P<0.05). Compared with control group, the density of L-type calcium channel current decreased in rapamycin group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + rapamycin group(P<0.05).CONCLUSION: Autophagy may be involved in the gp120V3 loop-mediated L-type calcium channels in hippocampus neurons.