三七总皂苷减少高脂血症兔动脉壁损害作用的实验研究

目的 观察三七总皂苷（PNS）减少高脂血症兔动脉壁的损害作用.方法 将健康新西兰兔随机分为正常对照组（NC）、动脉粥样硬化模型组（HC）、PNS中剂量治疗组（MD）及PNS高剂量治疗组（HD）,NC组喂饲普通饲料,后3组喂食1.5％高胆固醇饲料.PNS治疗组HD、MD分别采取静脉注射给药法经兔耳静脉注射PNS 100、50 mg/（kg·d）,于第3-4周第7-8周以及第11 -12周每隔14d给药PNS 3个疗程.于12周结束由耳缘静脉取血酶法测定血脂含量,摘取主动脉通过光镜和电镜观察病理学改变.结果 三七总皂苷能显著抑制血清总胆固醇（TC）,低密度脂蛋白胆固醇（LDL-C）水平的下降（P＜0.05）.结论 三七总皂苷对实验性动脉粥样硬化的形成有抑制作用.

Experimental Study on Protecting Effect of Total Saponins of Panax Notoginseng against Damage of Arterial Wall in Rabbit Caused by Hyper-lipemia

Objective To study the protecting effect of Total Saponins of Panax Notoginseng （PNS） on arterial wall in Hyperlipidemic rabbit model.Methods New Zealand rabbits were randomized into four groups：normal control group （NC） fed with common diet for 12 weeks; Hypercholesterolemia group （HC）,medium-dose PNS group （MD,PNS 50 mg/（kg·d） and high-dose PNS group （HD,PNS 100 mg/（kg·d）,fed with 1.5％ cholesterol for 12 weeks.In MD and HD groups,PNS was injected from 3th to 4th weeks for 2 weeks,7th to 8th weeks for 2 weeks,and 1 lth to 12th weeks for 2 weeks.The plasma levels of triglyceride （TG）,total cholesterol （TC）,low density lipoprotein cholesterol.（LDL-C） and high density lipoprotein cholesterol （HDL-C） were measured at the end of 12th week of experiment.Light microscopy and transmission electron microscopy were adopted to assess the degree of pathologic damage of arterial wall.Result PNS could reduce the levels of serum TC and LDL-C,as compared with the HC group,P＜0.05.Conclusion PNS has effect of inhibiting development of experimental atherosclerosis.