| 甲氧滴滴涕对人绒毛外滋养细胞的白细胞表面抗原G(HLA-G)表达的影响 |
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| 引用本文： | 侯蕾,陈必良,赵尹霄,赵莉莉.甲氧滴滴涕对人绒毛外滋养细胞的白细胞表面抗原G(HLA-G)表达的影响[J].生殖与避孕,2009,29(1). |
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| 作者姓名： | 侯蕾 陈必良 赵尹霄 赵莉莉 |
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| 作者单位： | 1. 96401部队537医院,宝鸡,721000
2. 第四军医大学西京医院妇产科,西安,710032 |
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| 摘 要： | 目的:探讨甲氧滴滴涕(MXC)对绒毛外滋养细胞(EVCT)白细胞表面抗原G(HLA-G)表达的影响。方法:用胰蛋白酶消化加流式细胞仪分离得到人绒毛外滋养细胞,进行原代培养及鉴定。将所获细胞分为MXC(1μmol/L、3μmol/L、5μmol/L、7μmol/L)处理组及空白对照组,培养12h、24h、48h。用Western blotting检测各组各时间点HLA-G的表达,同时用RT-PCR法检测HLA-GmRNA表达。结果:MXC处理组滋养细胞HLA-G表达呈时间剂量依赖性下降,HLA-G mRNA变化与其一致。结论:MXC可从蛋白和mRNA水平降低绒毛外滋养细胞HLA-G表达,从而降低其免疫耐受功能。
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| 关 键 词： | 甲氧滴滴涕(MXC) 绒毛外滋养细胞(EVCT) 白细胞表面抗原G(HLA-G) |
Effests of Myethoxychlor on the HLA-G Expression of Human Extravillous Trophobist |
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| Lei HOU,Bi-liang CHEN,Yin-xiao ZHAO,Li-li ZHAO.Effests of Myethoxychlor on the HLA-G Expression of Human Extravillous Trophobist[J].Reproduction and Contraception,2009,29(1). |
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| Authors: | Lei HOU Bi-liang CHEN Yin-xiao ZHAO Li-li ZHAO |
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| Institution: | 1. The 537 Hospital of Liberation 96401 Army; Baoji; 721000) (2. Department of Obstetrics and Gynecology; Xijing Hospital; Fourth Military Medical University; Xian; 710032); |
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| Abstract: | Objective:To observe the effect of methoxychlor(MXC) on the HLA-G of extravillous trophoblast in vitro. Methods:The extravillous trophoblast was incubated with 1 mmol/L,3 mmol/L,5 mmol/L,and 7 mmol/L MXC,collected affter at 12h,24h,48h. The effect of MXC on the expression of HLA-G and the change of HLAG mRNA in extravillous trophoblast was investigated by Western blotting and RT-PCR,respectively. Results:MXC can inhibit the extravillous trophoblast expression of HLA-G in a doseand time-dependent manner,and ... |
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| Keywords: | methoxychlor extravillous trophoblast HLA-G |
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