Claudin-1蛋白在甲状腺癌中的表达及调控

目的：研究Claudin-1蛋白在甲状腺癌中的表达。方法：选取27例乳头状甲状腺癌及其淋巴结转移组织、17例滤泡性甲状腺癌以及19例滤泡性腺癌患者的肿瘤组织,使用免疫组织化学方法检测Claudin-1蛋白的表达,并检测Claudin-1蛋白调控通路中β-catenin的表达。结果：乳头状甲状腺癌组织及转移淋巴结中均有Claudin-1蛋白的高表达,滤泡性甲状腺癌以及滤泡性腺癌患者的肿瘤组织中Claudin-1蛋白的表达较低。相关分析表明β-catenin的表达与Claudin-1蛋白的表达呈显著相关性,甲状腺癌中Claudin-1蛋白的表达可能受wnt/β-catenin途径进行调控。结论：Claudin-1蛋白在甲状腺癌不同类型中有不同的表达,是甲状腺肿瘤的一个重要标志物,其产生受wnt/β-catenin途径进行调控。

Expression and Regulation of Claudin-1 Protein in Thyroid Carcinoma

Objective：To determine the pattern of claudin-1expression in various types of thyroid lesions at the protein level and investigate the immunolocalization ofβ-catenin reported to regulate claudin-1expression.Methods：Tweenty-seven cases of papillary thyroid carcinoma patients,17follicular thyroid carcinomas patients and 19 follicular adenomas patients were evaluated by quantitative immunohistochemistry in claudin-1andβ-catenin expression.Results：All cases conspicuous claudin-1immunostaining was detected in the majority of papillary thyroid carcinoma and lymph node metastases（19/27and 9/10,respectively）.On the other hand,we found weak or no claudin-1expression in any of the follicular thyroid carcinomas and follicular adenomas cases.Conclusion：Our data prove that high claudin-1protein expression is an important marker of thyroid cancer,and the generation of claudin-1is regulated by wnt/β-catenin pathway in thyroid tumors.