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| 骨髓基质细胞移植对大鼠损伤脊髓GDNF mRNA表达的影响 | |
| 引用本文： | 李雷,吕刚,王岩峰,高红,许欣,白伦浩,王欢.骨髓基质细胞移植对大鼠损伤脊髓GDNF mRNA表达的影响[J].中国医科大学学报,2005,34(2):102-104. |
| 作者姓名： | 李雷 吕刚 王岩峰 高红 许欣 白伦浩 王欢 |
| 作者单位： | 1. 中国医科大学附属第二医院骨科,辽宁,沈阳,110004 2. 附属第一医院骨科 3. 附属第二医院小儿先天畸形实验室 4. 基础医学院发育生物学研究室 |
| 摘 要： | 目的:研究骨髓基质细胞(BMSCs)移植对大鼠损伤脊髓胶质细胞源性神经营养因子(GDNF)表达的影响.方法:将大鼠36只随机分为移植组和对照组,每组3个时相点,每个时相点每组6只动物.移植或手术后24,72 h和7 d,用RT-PCR方法对GDNF的表达进行半定量分析.结果:移植组GDNF mRNA 24,72 h和7 d时都明显高于单纯损伤组(P＜0.05).结论:BMSCs移植可使损伤脊髓表达GDNF增强. |
| 关 键 词： | 脊髓损伤 骨髓基质细胞 移植 神经营养因子 大鼠 |
| 文章编号： | 0258-4646(2005)02-0102-03 |
| 修稿时间： | 2004年10月8日 |
Influence of BMSCs transplantation on expression of GDNF mRNA in spinal cord injury of rats |
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| LI Lei,L Gang,WANG Yan-feng,GAO Hong,XU Xin,BAI Lun-hao,WANG Huan.Influence of BMSCs transplantation on expression of GDNF mRNA in spinal cord injury of rats[J].Journal of China Medical University,2005,34(2):102-104. | |
| Authors: | LI Lei L Gang WANG Yan-feng GAO Hong XU Xin BAI Lun-hao WANG Huan |
| Institution: | LI Lei,L(U) Gang,WANG Yan-feng,GAO Hong,XU Xin,BAI Lun-hao,WANG Huan |
| Abstract: | Objective: To explore the influence of bone marrow stromal cells(BMSCs)transplantation on expression of gliocyte derived neurotrophic factor(GDNF) mRNA in spinal cord injury of rats.Methods:Thirty-six rats were divided into transplantation group and control group.There were 3 time points in each group.The expression of GDNF mRNA were evaluated by using RT-PCR after transplantation or operation. Results:The expression levels of GDNF mRNA in transplantation group were significantly higher than those in control group at the 24-hour,72-hour,and 7-day time points.Conclusion:The transplantation of BMSCs can elevate the expression levels of GDNF mRNA in spinal cord injury of rats. |
| Keywords: | spinal cord injury bone marrow stromal cells transplantation neurotrophic factor rat |
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