自体骨髓单核细胞移植对兔肝内缺血型胆道病变的影响

目的 探讨自体骨髓单核细胞移植对血管新生及胆道缺血病变的影响.方法 将30只日本大耳白兔随机分为3组:假手术组(A组)、实验模型组(B组)和骨髓单核细胞移植组(C组),每组10只.3组均游离胆总管、肝总动脉及其间的疏松结缔组织,B组和C组阻断胆总管远端及肝总动脉2 h,C组经肝总动脉注射PKH26标记的自体骨髓单核细胞.术后监测生化指标的变化情况.术后4周开腹行胆道造影.同时,取第一肝门处汇管区肝组织制作石蜡切片,应用免疫组织化学方法观察自体骨髓单核细胞在肝内缺血环境中的分布及分化情况,并检测微血管密度.结果 术后C组生化指标恢复明显优于B组,骨髓单核细胞可分化成血管内皮细胞.术后4周,C组胆道破坏较B组轻,且胆道周围新生毛细血管多于B组.结论 自体骨髓单核细胞移植可以促进局部缺血组织毛细血管的增生,改善局部缺血胆道组织的微循环,从而减轻缺血型胆道病变的程度,甚至预防缺血型胆道病变的发生.

Effect of implantation of bone marrow mononuclear cells on ischemic-type intrahepatic biliary lesion in rabbits

Objective To investigate the effect of implantation of bone marrow mononuclear cells (BM-MNCs)on neovascularization and ischemic-type intrahepatic biliary lesion in rabbits.Methods The animals were divided into the sham-operation group, experimental model group and BM-MNCs implantation group with 10 rabbits in each. The animal model of ischemic-type intrahepatic biliary lesion was established by clamping the hepatic artery and common bile duct. The BM-MNCswere isolated from the tibial plateau by means of density gradient centrifugation and were implanted through the common hepatic artery. Changes of some biochemical markers such as ALT, AST, ALP,γ-GT, TBIL and DBIL etc. were detected. In 4 weeks after operation, the cholangiography, histopathological manifestation, differentiation of BM-MNCs, and microvessel density were observed.Results At each observation time, the degree of change of biochemical markers in group C was lower than that in group B. The engrafted cells could differentiate into vascular endothelial cells. The intrahepatic biliary lesion of group B was severer than that of group C but had fewer new capillary blood vessels around it. Conclusion The implantation of BM-MNCs can promote neovascularization and increase blood supply to the ischemic bile duct to diminish or prevent ischemic-type intrahepatic biliary lesion.