17-DMAG对人LX2肝星状细胞增殖及凋亡作用的研究

目的研究HSP90抑制剂17-DMAG在体外对人LX2肝星状细胞生长增殖及细胞凋亡的作用。方法 ?体外培养人LX2肝星状细胞,用细胞毒试验法(MTT)观察不同浓度和作用时间的17-DMAG对LX2细胞的生长抑制作用;流式细胞仪检测药物作用后LX2细胞凋亡的发生;Western blot检测α-SMA的表达,并与对照组进行对比。结果 17-DMAG能抑制人LX2肝星状细胞生长,且呈浓度和时间依赖性(P<0.01),随药物浓度加大和作用时间延长,存活细胞数量逐渐减少;流式细胞仪证实17-DMAG可诱导LX2细胞凋亡,与对照组比较,实验组细胞凋亡率明显增加(P<0.01);Western blot可以证实17-DMAG可使LX2细胞α-SMA表达减少,与对照组比较差异有统计学意义(P<0.05)。结论 17-DMAG可呈浓度和时间依赖性的抑制人LX2肝星状细胞的生长,其机制为诱导细胞凋亡,并且通过这种机制来减少α-SMA的表达来抑制胶原的产生。

Effect of 17-DMAG on proliferation and apoptosis of human hepatic satellite cell line LX2

Objective To investigate the effect of Heat Shock Protein 90（HSP90） inhibitor 17-DMAG on the proliferation and apoptosis of human hepatic satellite cell line LX2 cells in vitro. Methods The LX2 cells were cultured in vitro. The inhibitory effect of 17-DMAG on LX2 cells in different time and concentration was assayed by MTT assay. Flow cytometry was used to test the apoptosis of 17-DMAG interfered cells. The expression of α-SMA protein was detected by Western blot and compared with control group. Results The results showed that 17-DMAG could inhibit the growth of LX2 cells in a time and concentration dependent manner（P〈0.01）. With the increase of concentration of 17-DMAG, and the time interfered, the amount of live cells decreased. The apoptosis effect of 17-DMAG on LX2 cells was confirmed by flow cytometry, compared with control group, the apoptosis rate of the interfered group increased obviously（P〈0.01）. Western blot showed that 17-DMAG could decreased the expression of α-SMA. Conclusion 17-DMAG could inhibit the proliferation of LX2 cells in a concentrate and time dependent manners by inducing their apoptosis, and later decrease the expression of α-SMA to inhibit the production of collagen.