Egr-1基因剔除减少炎性相关因子在实验性胰腺炎中的表达

目的：探讨Egr-1基因剔除对实验性胰腺炎小鼠胰腺组织中炎性相关因子表达的影响。 〖HTH〗方法：利用Egr-1基因剔除小鼠，采用大剂量雨蛙素诱导的实验性胰腺炎模型，观察Egr-1基因剔除后，胰腺组织水肿、MPO水平、血清淀粉酶水平、肺组织MPO水平的改变。并利用定量PCR的方法，检测胰腺组织中炎症相关因子组织因子（TF）、纤维蛋白溶酶原激活因子抑制因子（PAI-1）、单核细胞趋化吸引蛋白1（MCP-1）、Gro-1、IL-6和细胞间黏附分子-1（ICAM-1） mRNA的表达。 〖HTH〗结果：Egr-1基因剔除小鼠胰腺组织水肿明显轻于野生型，但组织MPO水平与血清淀粉酶与野生型组间相比无明显差异；肺组织MPO水平明显低于野生型。定量PCR检测结果表明， Egr-1基因剔除组，胰腺组织中TF、PAI-1，以及MCP-1、ICAM-1和IL-6　mRNA的表达，明显少于野生型组。 〖HTH〗结论：Egr-1基因剔除可明显减轻急性胰腺炎的严重程度，其作用可能通过减少胰腺组织中TF、PAI-1，以及MCP-1、ICAM-1和IL-6的表达而实现。

Reduction of inflammatory-related factor expression in experimental acute pancreatitis in Egr-1 knockout mice

AIM: To observe the effects of Egr-1 gene knockout on the expression of inflammatory-related factors in pancreatic tissue in a mouse acute pancreatitis model.METHODS: The experimental pancreatitis was induced by high-dose of cearulein in wildtype mice and Egr-1 knockout mice.The pancreatitis indexes,such as serum amylase,pancreata edema,and myeloperoxidase(MPO) levels in pancreata and lungs were recorded.The mRNA levels of tissue factor(TF),plasminogen activator inhibitor(PAI-1),monocyte chemoattractant protein(MCP-1),Gro-1,IL-6 and ICAM-1 were measured by quantitative PCR.RESULTS: Contrary to wildtype mice,typical pancreatitis was not induced by high-dose cearulein in the Egr-1 knockout mice,not only markedly reduced edema in pancreata and lungs,but decreased MPO levels in lungs as well were found.Furthermore,the mRNA of TF,PAI,MCAP,ICAM-1 and IL-6 in pancreata were significantly decreased in Egr-1 knockout mice.CONCLUSION: The severity of pancreatitis and lung damage is ameliorated in Egr-1 knockout mice stimulated by high-dosage of cearulein,which was probably mediated by decreasing expression of inflammatory-related factors in pancreata,such as TF,PAI,MCP-1,ICAM-1 and IL-6.