成年和老年大鼠局灶性脑缺血后室管膜下区细胞增殖的比较研究

目的：比较老年和成年大鼠局灶性脑缺血后室管膜下区(the subventricular zone，SVZ)神经干细胞的增殖情况。方法：制作大脑中动脉梗塞模型，用免疫组织化学方法动态检测室管膜下区5-溴脱氧尿嘧啶(bromndeoxyuridine，BrdU)标记的阳性细胞数的变化：结果：正常组和假手术组成年大鼠SVZ的BrdU阳性细胞明显多于老年大鼠。实验组成年和老年大鼠SVZ的BrdU阳性细胞均在缺血后3d开始增加，7d时达到高峰，28d时仍高于正常水平，且成年大鼠各时间点SVZ的BrdU阳性细胞均明显高于老年大鼠。结论：大鼠脑缺血激活SVZ神经干细胞增殖，成年大鼠干细胞增殖能力强于老年大鼠。

Proliferation of neural stem cells in the subventricular zone in adult and aged rats after focal cerebral ischemia

Objective To compare the proliferation of neural stem cells in the subventricular zone (SVZ) in adult and old rats with focal cerebral ischemia.Methods In middle cerebral artery occlusion (MACO) models, bromodeoxyuridine (BrdU) positive cells in SVZ were observed with immunohistochemistry.Results In nonischemia rats,BrdU positive cells in SVZ of the adult are much more than those of the old. BrdU positive cells in SVZ of MCAO rats were obviously increased,and BrdU positive cells in SVZ of adult rats outnumbered those of old rats on every time point.Conclusion Neural stem cells in SVZ activated after cerebral ischemia may be related to recovery of neural cells and function after cerebral ischemia. Neural stem cells in SVZ of adult rats proliferates more than that of old rats.