河南省汉族人群基质金属蛋白酶-1基因-519A/G多态性及其血浆水平与急性冠状动脉综合征的相关性分析

目的:探讨河南省汉族人群中基质金属蛋白酶-1(MMP-1)基因-519A/G多态性及其血浆水平与急性冠状动脉综合征(ACS)的相关性。方法:采用多聚酶链反应-限制性片断长度多态性分析(PCR-RFLP)结合琼脂糖凝胶电泳技术检测295例ACS患者、105例稳定型心绞痛(SAP)患者和198例健康对照者MMP-1基因-519A/G多态性;采用酶联免疫吸附法(ELISA)测定血浆MMP-l水平,比较2组之间MMP-1基因多态性频率分布的差异和血浆水平的差异。结果:①ACS组MMP-1基因AA型所占比例明显高于SAP组及对照组,差异具有统计学意义(P<0.05);A等位基因频率在ACS组、SAP组和对照组分别为90.5%、83.3%、83.6%,ACS组与SAP组和对照组比较,差异亦有统计学意义(P<0.05)。②ACS组血浆MMP-l水平高于SAP组和对照组(P<0.05)。③不同基因型之间血浆MMP-1水平差异无统计学意义。结论:MMP-1基因-519A/G多态性可能与河南省汉族人群ACS发病相关;血浆MMP-l水平与冠状动脉斑块不稳定性相关。

Study on the relationship between the serum level and gene polymorphism of matrix metalloproteinase-1 and acute coronary syndrome in the Han nationality from Henan province

Objective:To investigate the relationship between the serum level and gene polymorphism of matrix metalloproteinase-1(MMP-1) and acute coronary syndrome(ACS) in the Han nationality from Henan province.Method:Two hundred and ninety nine patients with ACS,105 patients with SAP and 198 normal controls were enrolled and examined by the method of polymerase chain reaction-restriction fragment length poly-morphism(PCR-RFLP) for the-519 A/G polymorphism of MMP-1 gene.The serum level of MMP-1 was measured by enzyme...