环孢霉素A对小鼠自然流产模型外周CD4~+CD25~+T细胞及妊娠结局的影响

为探讨着床期应用环孢霉素A(CsA)对小鼠自然流产模型外周CD4+CD25+T细胞及其妊娠结局的影响,以CBA/J♀×DBA/2♂为自然流产模型,在孕第4天给孕鼠单次口服不同剂量的CsA。孕第14天计数胚胎吸收率;并采用流式细胞术分析孕鼠脾脏CD4+CD25+T细胞比例。结果表明:与自然流产模型孕鼠比较,于着床期给予0.1 mg/kg、1 mg/kg、10mg/kg CSA,可显著降低自然流产模型胚胎吸收率(P<0.05)。在应用CsA后,自然流产模型孕鼠脾脏CD4+CD25+细胞占CD4+T细胞的比例显著增加(P<0.05)。表明CsA可通过上调外周CD4+CD25+T细胞,从而诱导母胎免疫耐受,改善妊娠预后。

Effects of cyclosporin A on the peripheral CD4+CD25+T cells and the outcomes of gestation in abortion-prone matings

To explore effect of cyclosproin A(CsA) on the peripheral CD4+CD25+T cells and pregnant outcomes in abortion-prone matings,after the abortion-prone CBA/J♀×DBA/2♂matings were established,the pregnant CBA/J females were treated with 0 mg/kg,0.1 mg/kg,1 mg/kg,10 mg/kg CsA by oral at day 4 of gestation(the window of murine implantation),respectively.The embryo resorbing rate was counted,and proportion of CD4+CD25+T cells in splenic CD4+ T cells were analyzed by flow cytometry(FCM) at day 14 of gestation.After treatment with 0.1 mg/kg,1 mg/kg,10 mg/kg CsA in the abortion-prone model,the proportion of CD4+CD25+T cells in splenic CD4+T cells increased sigificantly(P<0.05),which was beneficial for the outcomes of pregnancy,and the embryo resorbing rats was reduced remarkably(P<0.05,P<0.01,P<0.05).It is concluded CsA can induce materno-fetal immune tolerance by up-regulating proportion of peripheral CD4+CD25+T cells,which result in decrease of the embryo resorbing rate in abortion-prone matings.