我国北方地区小儿哮喘CTLA-4基因多态性的相关研究

目的:探讨细胞毒T淋巴细胞相关抗原4(CTLA-4)启动子-318C/T基因多态性与我国北方地区儿童哮喘的关系及其临床意义。方法:采用PCR-RFLP法检测90例哮喘患儿和100例健康儿童的CTLA-4-318C/T的基因多态性。结果:CTLA-4-318位点存在基因多态性;重度哮喘组基因型分布和T等位基因频率明显高于轻、中度哮喘组及正常对照组,具有显著性差异(P均<0.05)。结论:CTLA-4-318位点存在基因多态性,T等位基因可能是重度哮喘的候选基因。

Study on cytotoxic T lymphocyte associated antigen 4 genetic polymorphisms in asthmatics of the children in northern China

Objective:To explore the relationship and clinical significance of cytotoxic T lymphocyte associated antigen 4(CTLA-4)genetic polymorphisms in asthmatic children in the northern China.Methods:PCR-RFLP technique was used to locate-318(C/T)of CTLA-4 genetic polymorphisms in 90 asthmatic children and in 100 healthy children from northern China.Results:The genetic polymorphisms of CTLA-4-318(C/T)was existed.Both the genotype distributions and allele frequencies in the severe asthmatic group were surpassed than those in the mild group,the moderate group and the normal controller,with prominent differences(all P<0.05).While either of the genotype distribution or of the allele frequencies,no significant differences was found during the mild,moderate asthmatic group and the normal controller group(all with P>0.05).Conclusion:The polymorphisms of CTLA-4 gene promoter-318 site are existed,and T allele frequencies may be a candidate gene of severe asthma.