电针次髎/中髎穴抑制膀胱过度活动的穴位特异性研究

目的探讨次髎和中髎穴抑制膀胱过度活动的穴位特异性。方法 60只SD大鼠随机分为模型组和5个电针组：次髎/中髎组、委中组、三阴交组、通天组、合谷组,每组10只。各组大鼠均先采用生理盐水进行膀胱灌注（基线Ⅰ）,继以0.25%乙酸膀胱灌注建立大鼠膀胱过度活动模型（基线Ⅱ）。造模成功后,5个电针组进行电针治疗5分钟,模型组不予电针治疗。电针后再次以生理盐水进行膀胱灌注。观察灌注期间大鼠排尿间隔时间、膀胱排尿压力、膀胱基础压力和平均排尿压力的改变。结果与基线Ⅰ相比,基线Ⅱ时各组排尿间隔时间显著缩短（P〈0.01）、膀胱排尿压力和平均排尿压力显著下降（P〈0.01）。电针干预后,各组排尿间隔时间较基线Ⅱ均显著延长,仅次髎/中髎组与基线Ⅰ比较差异不显著,并且其增加值显著大于模型组（P〈0.001）和其他电针组（P〈0.01,或P〈0.05）。次髎/中髎组电针后膀胱排尿压力较基线Ⅱ显著升高（P〈0.05）、与基线Ⅰ差异不显著,并显著高于模型组（P〈0.001）和其他电针组（P〈0.05）。结论次髎、中髎可有效抑制膀胱过度活动,此抑制效应具有穴位特异性。

Electroacupuncture at BL32 /BL33 in the inhibition of overactive bladder and its acupoints specificity

Objective To investigate the acupoints specificity of Ciliao（ BL32） and Zhongliao（ BL33） on inhibiting overactive activity of bladder. Methods 60 SD rats were randomly divided into 1 model group and 5 electroacupuncture groups： Ciliao / Zhongliao（ BL32 / BL33） group,Weizhong（ BL40） group,Hegu（ LI4） group,Tongtian（ BL7） group and Sanyinjiao（ SP6） group. Rats in each group were performed with bladder perfusion with normal saline（ basalⅠ,to get the normal data of rats） and 0. 25% Acetic acid（ basalⅡ,to make rat model of overactive bladder）,respectively. Rats in 5 electroacupunc- ture groups received elecltroacupuncture treatment 5 minutes,while rats in model group received no inter- vention. Rats in each group were performed with bladder perfusion with normal saline again. Changes of in- tercontraction interval（ ICI）,vesical micturition pressure（ VMP）,basal pressure and average voiding pressure in all groups were observed. Results Compared with basalⅠ,the ICI of each group was signifi- cantly shortened（ P〈0. 01） and the VMP significantly decreased（ P〈0. 01） at basalⅡ. After electroa- cupuncture treatment,the ICI were prolonged in all groups but also shorter than basal Ⅰ except Ciliao / Zhongliao group,while the increased value of Ciliao / Zhongliao group significantly greater than model group（ P〈0. 001） and other electroacupuncture groups（ P〈0. 01 or P〈0. 05）. The VMP of Ciliao/Zhongliao group was significantly increased（ P〈0. 05） compared with the model group（ P〈0. 001） and other elec- troacupuncture groups（ P〈0. 05）. Conclusion Electroacupuncture at Ciliao / Zhongliao point can inhibit bladder hyperactivity,which shows an effect of acupoints specificity.