视网膜神经节细胞体外高压培养模型的建立

目的建立视网膜神经节细胞的体外高压培养模型并观察压力和加压时间对视网膜神经节细胞凋亡的影响。方法建立视网膜神经节细胞混合培养和纯化培养的高压模型,并用流式细胞仪(FCM)检测凋亡细胞百分率。结果成功分离并培养了视网膜神经节细胞R(GCs)混合培养和纯化培养模型,RGCs的混合培养最长可存活3～6w,RGCs的纯化培养可存活时间4～7d。成功对混合培养和纯化培养的细胞进行压力造模,压力20、40、60、80mmHg,加压时间1～4h均能促进RGCs的凋亡,其凋亡细胞的数目以80mmHg、4h为最多(P<0.01)。结论混合培养的RNCs比纯化培养的RGCSs存活时间长。压力能促进RGCSs的凋亡,其凋亡细胞的数目随压力的增大、加压时间的延长而增加。

Establishment of Retinal Ganglion Cells Model with Hyperbaric Culture in Vitro

Objective To establish a hyperbaric model of Retinal Ganglion Cells(RGCs) in vitro and observe the effect of prassure and time on RGCSs.Methods Hyperbaric model of RGCSs was established with mixed and purity culture in votro,and the sum of apoptosis cells was measured with flow cytometry(FCM).Results RGCs was seperated successfully and RGCs model was cultured by mixed and purify methods in votro.RGCs could survive 3-6 weeks in mixed model and 4-7 days in purify model.The apoptosis in the hyperbaric model was promoted under the pressure of 20,40,60 and 80 mmHg,and during pressor time of 1-4 hour,and the volume of apoptosis was the most under the 80 mmHg at 4th hour(P<0.01).Conclusions The surviving time of RGCs in the mixed culture is longer than that in the purify.The pressure can promote the apoptosis of RGCs and the sum of apoptosis can increase due to the increasing pressure and the prolonging time.