自发性2型糖尿病大鼠肺组织α平滑肌肌动蛋白表达的变化及罗格列酮对其影响

目的 探讨自发性2型糖尿病(OLETF)大鼠肺组织α-平滑肌肌动蛋白(α-SMA)表达的变化及罗格列酮对其影响.方法 30周龄雄性OLETF大鼠16只,分为OLETF组和OLETF/L组每组8只,对照组为8只LETO组大鼠.OLETF/L组大鼠给予罗格列酮3 mg/(kg·d)灌胃12周后处死大白鼠,应用免疫组织化学和免疫蛋白印迹法检测各组大鼠肺组织α-SMA蛋白表达.结果 各组大鼠肺组织α-SMA主要表达于肌成纤维细胞、血管平滑肌细胞、小气道平滑肌,OLETF组大鼠肺组织α-SMA蛋白表达(2.03±0.64)明显高于LETO组(1.18±0.35),P<0.05];OLETF/L组明显减少(1.34±0.40),差异均有统计学意义(均P<0.05).结论 OLETF大鼠肺组织肌成纤维细胞生成增多,罗格列酮可抑制成纤维细胞向肌成纤维细胞的转化,早期改善糖尿病肺纤维化.

Abstract：

Objective To investigate the changes of expression of α—smooth muscle actin(α-SMA)in lung of spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty(OLETF)rats and the effect by tlle rosiglitazone.Methods Totally 16 male OLETF rats in 30 weeks old were divided into OLETF group and OLETF／L group.The Long Evans Tokushima Otsuka(LETO)rats were in control group.Thle OLtErI’F.rats were given gastric lavage bymsiglitazone 3 ms／(kg·d)then were killed after 12 weeks.The levels of ct-SMA in lung of three groups were detected by immunohistochemistry and western blotting.Results α-SMA in lung of three groups mainly expressed in myofibmblast.vascular smooth muscle cells and small airway smootII muscle.Compared to control group，tlle levels of α-SMA in lung of OIJETF group were increased obviously(2.03-4-0.64 VS 1.18 4-0.35，P<0.05).Compared to OLETF group，the levels of α-SMA in lung of OLETF／L group were decreased obviously(1.34 ±0.40 VS 2.03 ±0.64.P<0.05).Conclusion Myofibroblast increases in lung of OLETF rats.Rosiglitazone can inhibit the transformation from fibroblast to myofibroblast and improve pulmonary fibrosis in diabetes in early stage.