| 两性霉素B脂质体粒度测定方法研究 |
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| 引用本文： | 许波,田颂九,吴如金,王国荣.两性霉素B脂质体粒度测定方法研究[J].药物分析杂志,2004,24(3):307-309. |
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| 作者姓名： | 许波 田颂九 吴如金 王国荣 |
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| 作者单位： | [1]贵州省药品检验所贵阳550004 [2]中国药品生物制品检定所,北京100050 [3]中国药科大学,南京210009 [4]国家药典委员会,北京100061 |
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| 摘 要： | 目的：建立两性霉素B脂质体粒度检测方法，通过测定一组性质不同的样品，找出最佳测定方法。方法：用计算机的图像一数字处理技术结合扫描电镜、透射电镜和激光光散射粒度测定仪分别测定两性霉素B脂质体的粒度。结果：电镜法测定两性霉素B脂质体的粒度为20—100nln，平均粒径为55—75nm；激光光散射法测定两性霉素B脂质体的粒度为30—200nm，平均粒径为50—180nm。结论：激光光散射法能较好反映两性霉素B脂质体在使用时的真实粒度，且方法快速、简便，是一种较好的两性霉素B脂质体粒度测定方法。
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| 关 键 词： | 两性霉素B脂质体粒度 测定方法 激光微粒测定仪 透射电镜 扫描电镜 多烯类抗生素 |
Study on the Method for Determination of the Particle Size of Liposome Amphotericin B |
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| XU bo,TIAN Song -jiu,WU Ru -jin and WANG Guo -rong
National Institute for the Control of Pharmaceutical and Biological Product,Beijing ,Guizhou Province Institute for Drug Control,Guiyang ,China Pharmaceutical University,Nanjing , State Pharmacopoeia Commission of China,Beijing.Study on the Method for Determination of the Particle Size of Liposome Amphotericin B[J].Chinese Journal of Pharmaceutical Analysis,2004,24(3):307-309. |
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| Authors: | XU bo TIAN Song -jiu WU Ru -jin and WANG Guo -rong
National Institute for the Control of Pharmaceutical and Biological Product Beijing Guizhou Province Institute for Drug Control Guiyang China Pharmaceutical University Nanjing State Pharmacopoeia Commission of China Beijing |
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| Institution: | XU bo,TIAN Song -jiu,WU Ru -jin and WANG Guo -rong
National Institute for the Control of Pharmaceutical and Biological Product,Beijing 100050,Guizhou Province Institute for Drug Control,Guiyang 550004,China Pharmaceutical University,Nanjing 210009, State Pharmacopoeia Commission of China,Beijing 100061 |
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| Abstract: | Objective:To establish the method for the determination of the particle size of liposome amphotericin B. Method:Determination of the particle size of liposome Amphotericin B by scanning electron microscopy,transmission electron microscopy and the laser scattering particle size distribution analyzer. Results:Particle size of liposome amphotericin B is 20-100 nm and the average size is 55 - 75 nm, which are determined by electron microscopy; the particle size of liposome amphotericin B is 30 -200 nm and the average size is 50 - 180 nm,which are determined by laser scattering particle size distribution analyzer. Conclusion: The laser scattering particle size distribution analyzer is rapid,simple and accurate for the determination of the particle size of liposome amphotericin B. |
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| Keywords: | liposome amphotericin B scanning electron microscopy transmission electron microscopy laser scattering particle size distribution analyzer |
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