| 雄激素干预对PC12细胞Seladin-1表达的影响 |
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| 引用本文： | 邬军锋,祖衡兵,陈英辉,张建峰,王翠翠,王子高.雄激素干预对PC12细胞Seladin-1表达的影响[J].神经疾病与精神卫生,2010,10(2):119-121. |
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| 作者姓名： | 邬军锋 祖衡兵 陈英辉 张建峰 王翠翠 王子高 |
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| 作者单位： | 复旦大学附属金山医院神经内科,200540 |
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| 摘 要： | 目的观察雄激素干预对体外培养PC12细胞Seladin-1水平的影响。方法将培养的PC12细胞分为5组：正常对照组、药物处理组（雄激素终浓度分别为1×10-3mol/L、1×10-4mol、1×10-5mol/L、1×10-6mol/L）。采用Western-blot和Real-time PCR方法对PC12细胞Seladin-1的表达进行检测。结果各药物组处理后，PC12细胞Seladin-1的蛋白质与mRNA的表达水平与正常对照组比较均明显提高，差异具有统计学意义（P〈0.05，P〈0.001）。结论雄激素对PC12细胞Seladin-1基因的表达具有正性调控作用。雄激素的神经保护效应可能与上调seladin-1的表达有关。
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| 关 键 词： | PC12细胞 雄激素类 Seladin-1 基因表达 |
Effects of androgen on the expression of Seladin-1 in PC12 cells |
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| Institution: | WU Jun-feng, ZU Heng-bing, CHEN Ying-hui, et al. Department of Neurology, the Affiliated Jinshan Hospital of Fudan Uni- versity, Shanghai 200540, China |
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| Abstract: | Objective To investigate the effects of androgen on the expression of Seladin--1 in PC12 cells. Methods The effects of different concentrations of androgen on the expression of Seladin--1 in PC12 cells were determined by Western blot analysis and Real--time PCR. Results The expression of Seladin--1 was significantly increased in the levels of both protein and mRNA （P 〈 0.05, P 〈 0. 001）. Conclusions Androgen can positively modulate the expression of Seladin--1. The neuroprotective effect of androgen might be mediated by the up-- regulation of Seladin-- 1 expression. |
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| Keywords: | Seladin-1 |
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