| 干扰素对肿瘤细胞Fas表达的上调作用及与细胞凋亡的关系 |
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| 摘 要： | ![]()
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| 关 键 词： | 肿瘤细胞 Fas表达 细胞凋亡 干扰素γ CD95抗原 脱噬作用 基因表达调控 流式细胞术 酶联免疫吸附测定 |
Interferon-gamma up-regulates Fas expression and increases Fas-mediated apoptosis in tumor cell lines] |
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| Hang Zheng,Rong-Cheng Luo,Luo-Sheng Zhang,Guo-Feng Mai. Interferon-gamma up-regulates Fas expression and increases Fas-mediated apoptosis in tumor cell lines][J]. Journal of First Military Medical University, 2002, 22(12): 1090-1092 |
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| Authors: | Hang Zheng Rong-Cheng Luo Luo-Sheng Zhang Guo-Feng Mai |
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| Affiliation: | Department of Oncology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | ![]()
OBJECTIVE: To investigate effect of interferon-gamma (IFN-gamma) on Fas expression and Fas-mediated apoptosis in tumor cell lines. METHODS: Fas expression was detected by flow cytometry, and the tumor cell apoptosis evaluated by enzyme-linked immunosorbent assay and AnnexinV staining assay. RESULTS: All the 4 tumor cell lines used in this study, e.g. gastric cancer cell lines Kato-III AGS, lung cancer cell line A549 and laryngeal cancer cell line Hep-2, expressed Fas protein to varied degrees. Fas expression was up-regulated by IFN-gamma (P<0.05), which alone was enough to induce apoptosis in tumor cells and increase the susceptibility of them to anti-Fas antibodies. IFN-gamma induced Fas expression up-regulation and tumor cell apoptosis in a time-dependent manner. CONCLUSION: Fas-mediated apoptosis is one of the mechanisms for IFN-gamma to exercise its anti-tumor effect. |
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