| 高效液相色谱法测定艾司唑仑片中艾司唑仑含量 |
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| 引用本文： | 杨惠霞,何文斌. 高效液相色谱法测定艾司唑仑片中艾司唑仑含量[J]. 中国药业, 2011, 20(21): 26-27 |
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| 作者姓名： | 杨惠霞 何文斌 |
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| 作者单位： | 1. 广东省佛山市中医院药剂科,广东佛山,528000
2. 广东省佛山市药品检验所,广东佛山,528000 |
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| 摘 要： | 目的探讨用高效液相色谱法测定艾司唑仑片含量的方法和条件。方法色谱柱为Kcromaisil ODS-1-C18柱(200 mm×4.6 mm,5μm);流动相为甲醇-水(60∶40),流速为1.0 mL/min;检测波长为223 nm;柱温为30℃。结果艾司唑仑进样量在0.103～2.06μg范围内线性关系良好(r=1.000 0),艾司唑仑平均回收率为99.73%,RSD=0.77%。结论该方法灵敏、专一、准确,适用于艾司唑仑片的含量测定。
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| 关 键 词： | 高效液相色谱法 艾司唑仑 含量测定 |
Determination of Estazolam in Estazolam Tablets by HPLC |
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| Yang Huixia,He Wenbin. Determination of Estazolam in Estazolam Tablets by HPLC[J]. China Pharmaceuticals, 2011, 20(21): 26-27 |
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| Authors: | Yang Huixia He Wenbin |
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| Affiliation: | Yang Huixia1,He Wenbin2(1.Department of Pharmacy,Foshan Hospital of TCM,Foshan,Guangdong,China 528000,2.Foshan Instituate for Drug Control,China 528000) |
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| Abstract: | Objective To investigate methods and conditions for the determination of estazolam in Estazolam Tablets by HPLC.Methods A Kcromaisil ODS-1-C18（200 mm×4.6 mm,5 μm） column was used with methanol-water（60 ∶40） as mobile phase.The flow rate was 1.0 mL/min and the column temperature was set at 30 ℃.The UV detection wavelength was 223 nm.Results The linear range for estazolam was 0.103-2.06 μg（r=1.000 0）.The average recovery rate was 99.73%,RSD=0.77%（n=5）.Conclusion The method was sensitive,specific and accurate,and can be used for the determination of estazolam in Estazolam Tablets. |
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| Keywords: | HPLC estazolam content determination |
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