祁州漏芦不同溶剂提取物对急性肝损伤模型小鼠的保护作用

目的:研究祁州漏芦(以下简称"漏芦")不同溶剂提取物对急性肝损伤模型小鼠的保护作用。方法:60只KM种小鼠随机分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、联苯双酯(100 mg/kg)组、漏芦乙醇粗提物(200 mg/kg)组、漏芦正丁醇提取物(200 mg/kg)组和漏芦水提取物(200 mg/kg)组。灌胃给药,每天1次,连续7 d。末次给药1 h后,一次性腹腔注射300 mg/kg对乙酰氨基酚(APAP)以复制小鼠急性肝损伤模型。比色法检测小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、白蛋白(ALB),肝匀浆丙二醛(MDA)、还原型谷胱甘肽(GSH)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST),肝线粒体Na+-K+-ATP酶(Na+-K+-ATPase)、Ca2+-Mg2+-ATP酶(Ca2+-Mg2+-ATPase)水平。结果:与正常对照组比较,模型组小鼠血清ALT、AST和ALP活性增强,ALB含量降低;肝组织MDA含量增加,CAT、GPx、SOD、GST活性减弱,GSH含量减少;肝线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性减弱,差异具有统计学意义(P<0.05)。与模型组比较,漏芦乙醇粗提物组小鼠血清ALT、AST活性减弱,ALB含量增加,肝匀浆MDA含量减少,GSH含量增加,CAT、GPx、SOD、GST活性增强,肝线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性增强,差异具有统计学意义(P<0.05);漏芦正丁醇提取物组小鼠血清ALT、AST、ALP活性减弱,ALB含量增加,肝匀浆MDA含量减少,GSH含量增加,CAT、GPx、SOD、GST活性增强,肝线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性增强,差异具有统计学意义(P<0.05);漏芦水提取物组小鼠血清ALT、AST活性减弱,ALB含量增加,肝匀浆MDA含量减少,GSH含量增加,GPx、GST活性增强,肝线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性增强,差异具有统计学意义(P<0.05)。结论:漏芦不同溶剂提取物对APAP致小鼠急性肝损伤具有保护作用,其机制可能与其抗氧化作用有关。

Protective Effects of the Extracts ofRhaponticum uniflorum on Acute Liver Injury Model Mice

OBJECTIVE： To investigate the protective effect of the extracts of Rhaponticum uniflorum on acute liver injury model mice. METHODS： 60 KM mice were randomly assigned into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, bifendate group （100 mg/kg）, ethanol extract of R. uniflorum group （200 mg/ kg）, n-butanol extract ofR. uniflorum group （200 mg/kg） and aqueous extract ofR. uniflorum group （200 mg/kg）. They were given relevant medicines intragastrically once a day for consecutive 7 days. 1 h after last administration, acute liver injury model was induced by disposable intraperitoneal injection of APAP （300 mg/kg）. The colorimetric method was adopted to detect the levels of ALT, AST, ALP, ALB, MDA, GSH, CAT, GPx, SOD, GST, Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase. RESULTS： Compared with normal control group, the activities of ALT, AST and ALP in serum of model group increased significantly, while the content of ALB in serum decreased significantly; the content of MDA in liver tissue increased significantly while the activities of CAT, GPx, SOD and GST and the content of GSH in liver tissue decreased significantly; the activities of Na ＋-K ＋-ATPase and CaZ＋-Mg2＋-ATPase in hepatic mitochondria decreased significantly（P〈0.05）. Compared with model group, the activities of ALT, AST in serum decreased significantly in ethanol extract of R. uniflorum group while the content of ALB in serum increased signifi- cantly; the contents of MDA in liver homogenate decreased significantly, while the activities of CAT, GPx, SOD and GST and the content of GSH increased significantly; the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in hepatic mitochondria increased sig- nificantly（P〈 0.05）. The activities of ALT, A ST and ALP in serum decreased significantly in ethanol extract of n-butanol extract of R. uniflorum group , while the content of ALB in serum increased significantly; the contents of MDA in liver homogenate decreased significantly, while the activities of CAT, GPx, SOD and GST and the content of GSH increased significantly; the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in hepatic mitochondria increased significantly（P〈：0.05）.The activities of ALT and AST in serum of aqueous extract of R. uniflorum group decreased significantly, while the content of ALB in serum increased significantly;the content of MDA in liver homogenat decreased significantly, while the activities of GPx and GST and the content of GSH in liver homogenate increased significantly; the activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in hepatic mitochondria increased significantly （P〈0.05）. CONCLUSIONS： R.uniflorum extracts have protective effects on acute liver injury induced by APAP in mice, which is probably associated with anti-oxidative activities.