|
|||||
|
|
| 碳纳米管携载顺铂逆转卵巢癌耐药性的实验研究 | |
| 引用本文： | 彭芸花,贾学玲,孙绍伯,佥玉.碳纳米管携载顺铂逆转卵巢癌耐药性的实验研究[J].实用妇产科杂志,2011,27(10). |
| 作者姓名： | 彭芸花 贾学玲 孙绍伯 佥玉 |
| 作者单位： | 1. 兰州大学基础医学院遗传所,甘肃兰州730000;兰州大学第一医院,甘肃兰州730000 2. 兰州大学第一医院,甘肃兰州,730000 3. 甘肃省中医学院,甘肃兰州,730000 4. 兰州大学基础医学院遗传所,甘肃兰州730000;南京大学医学院,江苏南京210093 |
| 摘 要： | 目的:研究以荧光标记的大孔径碳纳米管为载体,内载顺铂,作用于顺铂耐药的卵巢癌细胞株,逆转细胞耐药性,增加顺铂对细胞的杀伤作用。方法:携带荧光标记物的碳纳米管内载顺铂后与卵巢癌耐药细胞株共培养,通过荧光显微镜检测碳纳米管载体进入细胞内的量,流式细胞仪检测载体增加顺铂对癌细胞的杀伤性能,高效液相色谱法检测载体增加细胞内顺铂含量。结果:①荧光显微镜检测结果:随着单壁碳纳米管(SWNTs)浓度(6μg/ml、12.5μg/ml、25μg/ml、50μg/ml、100μg/ml、200μg/ml)的增加,顺铂-SWNTs粒子进入卵巢癌耐药COC1/DDP细胞株的量也增加;②流式细胞仪检测:浓度高于1×10-3mg/ml时,顺铂对细胞产生了明显的凋亡效应;③高效液相色谱法检测:作用2小时后,100μg/ml和50μg/ml浓度加载体组细胞内顺铂含量(10.1478±1.2362)×10-6ng、(9.3208±1.4302)×10-6ng]比不加载体组细胞内顺铂含量(4.2835±0.7728)×10-6ng、(4.6852±1.0038)×10-6ng]明显增多(P<0.01)。结论:功能化的碳纳米管有望成为新的化... |
| 关 键 词： | 碳纳米管 药物载体 顺铂 耐药卵巢癌细胞 |
Enhancement of Anticancer Effects of Cisplatin Carried by Single-Walled Carbon Nanotubes to Human Ovarian Carcinoma Cisplatin-Resistant Cells |
|
| PENG Yunhua,JIA Xueling,SUN Shaobo,et al.Enhancement of Anticancer Effects of Cisplatin Carried by Single-Walled Carbon Nanotubes to Human Ovarian Carcinoma Cisplatin-Resistant Cells[J].Journal of Practical Obstetrics and Gynecology,2011,27(10). | |
| Authors: | PENG Yunhua JIA Xueling SUN Shaobo |
| Institution: | PENG Yunhua1,2,JIA Xueling2,SUN Shaobo3,et al(1.Genetic Institute,School of Basic Medicine,Lanzhou University,Lanzhou Gansu 730000,China,2. The First Hospital of Lanzhou University,3.College of Traditional Chinese Medicine,Nanjing Jiangsu 210093,China) |
| Abstract: | Objective:To study the anticancer potential of single-walled carbon nanotubes(SWNTs) loaded with cisplatin and rhodamine in human ovarian carcinoma cisplatin-resistant cells.Methods:Cisplatin carried by SWNTs was co-cultured with human ovarian carcinoma cisplatin-resistant cells. The dosage of SWNTs vector entered into cells was observed by fluorescence microscopy The cytotoxic effect of cisplatin-loaded SWNTs on the proliferation of human ovarian carcinoma cisplatin-resistant cells was observed by flow cyt... |
| Keywords: | Single-walled carbon nanotubes Drug agent Cisplatin Human ovarian carcinoma cisplatin-resistant cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |