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| 稀释碘伏对人胃癌细胞SGC-7901生长的影响 | |
| 引用本文： | 赵菊梅,刘勇峰,陈美霓,王爱红,庞秋霞,米志宽,魏晓丽. 稀释碘伏对人胃癌细胞SGC-7901生长的影响[J]. 山西医科大学学报, 2011, 42(4): 285-288,361. DOI: 10.3969/J.ISSN.1007-6611.2011.04.003 |
| 作者姓名： | 赵菊梅 刘勇峰 陈美霓 王爱红 庞秋霞 米志宽 魏晓丽 |
| 作者单位： | 1. 延安大学医学院医学实验中心,延安,716000 2. 延安大学附属医院普外科 |
| 基金项目： | 延安市科学技术研究资助项目; 陕西省卫生厅科研基金资助项目(2008H039) |
| 摘 要： | 目的探讨稀释碘伏对体外培养胃癌细胞SGC-7901生长的影响。方法培养人胃癌细胞SGC-7901,分别用0.5%,0.25%,0.01%浓度的碘伏处理对数生长期的胃癌细胞,采用噻唑蓝(MTT)法观察不同浓度碘伏对细胞增殖作用的影响,应用碘化丙锭(PI)、Hoechst33528染色观察其对肿瘤细胞影响的形态学改变,流式细胞技术检测肿瘤细胞的凋亡率。结果三种浓度(从低到高)碘伏均对胃癌细胞SGC-7901具有明显的抑制作用,抑制率分别为89.3%,88.5%和88.1%;0.01%的碘伏处理1 min的细胞,碘化丙锭(PI)、Hoechst33528染色观察到细胞凋亡的细胞皱缩、胞核浓缩、裂解等形态学改变;流式细胞仪结果显示0.01%碘伏处理细胞1 min即可诱导凋亡的发生。结论稀释碘伏对胃癌细胞SGC-7901具有明显的杀伤作用,为稀释碘伏预防肿瘤的种植转移提供了一定的理论依据。 |
| 关 键 词： | 聚维酮碘 胃癌 生长抑制 |
Povidone-iodione inhibited the growth of human gastric cancer cell line SGC-7901 |
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| ZHAO Ju-mei,LIU Yong-feng,CHEN Mei-ni,WANG Ai-hong,PANG Qiu-xia,MI Zhi-kuan,WEI Xiao-li. Povidone-iodione inhibited the growth of human gastric cancer cell line SGC-7901[J]. Journal of Shanxi Medical University, 2011, 42(4): 285-288,361. DOI: 10.3969/J.ISSN.1007-6611.2011.04.003 | |
| Authors: | ZHAO Ju-mei LIU Yong-feng CHEN Mei-ni WANG Ai-hong PANG Qiu-xia MI Zhi-kuan WEI Xiao-li |
| Affiliation: | ZHAO Ju-mei1,LIU Yong-feng2,CHEN Mei-ni1,WANG Ai-hong1,PANG Qiu-xia1,MI Zhi-kuan1,WEI Xiao-li1(1Medical Experiment Center,Medical College of Yan'an University,Yan'an 716000,China,2 Dept of General Surgery,First Affiliated Hospital of Yan'an University,) |
| Abstract: | Objective To investigate the effects of diluted povidone-iodione on proliferation and apoptosis of gastric cancer cell line SGC-7901.Methods Human gastric cancer cell line SGC-7901 cells were treated with different concentrations(0.5%,0.25%,0.01%) of diluted povidone-iodione,respectively.MTT assay was used to examine the proliferation.PI and Hoechst33528 staining were used to observe the morphological changes of cancer cells,and flow cytometry was performed to detect the apoptotic rate.Results The prolifera... |
| Keywords: | povidone-iodione gastric cancer growth inhibition |
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