| 瑞芬太尼预处理对孕鼠离体心肌缺血再灌注损伤的保护作用研究 |
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| 引用本文： | 吴铭广,李元涛,黄晓雷,肖永,王亮,黄穗萍. 瑞芬太尼预处理对孕鼠离体心肌缺血再灌注损伤的保护作用研究[J]. 陕西医学杂志, 2011, 40(1): 18-21 |
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| 作者姓名： | 吴铭广 李元涛 黄晓雷 肖永 王亮 黄穗萍 |
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| 作者单位： | 南方医科大学附属深圳妇幼保健院麻醉科,深圳,518028 |
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| 摘 要： | 目的:探讨孕鼠离体心脏对缺血再灌输损伤的耐受及不同浓度瑞芬太尼预处理对孕鼠离体心功能及心肌缺血再灌注损伤的保护作用。方法:将雌性SD大鼠40只中非孕鼠10只做为空白对照组,其余孕鼠30只,于孕后第20天随机分为3组:无瑞芬太尼预处理组(RF0组)、低浓度瑞芬太尼组(RF1组)和高浓度瑞芬太尼组(RF2组),每组10只。麻醉后开胸取心脏,建立Langendorff孕鼠离体心脏灌注模型。每组均灌注平衡20min,对照组(Con组)与无瑞芬太尼预处理组(RF0组)用95%O2和5%CO2饱和的K-H液持续灌注20min,停灌30min再灌注45min;浓度瑞芬太尼组(RF1组)、高浓度瑞芬太尼组(RF2组)缺血前分别用含瑞芬太尼20μg/L或40μg/L的95%O2和5%CO2饱和K-H液灌注20min,全心缺血30min,复灌45min;记录各组左室舒张末压(LVEDP),左室收缩压(LVSP)及冠脉流量(CF)变化,测定平衡15min后到再灌注后15min内冠脉流出液乳酸脱氢酶(LDH)活性,测定再灌注后45min时的心肌丙二醛(MDA)含量,同时取每组孕鼠心肌做切片,在电镜下观察心肌细胞的超微结构。结果:在平衡20min末,与对照组比较,各组LVEDP、LVSP和LDH的活性均无显著变化(P>0.05);与对照组比较,各组在灌注后各时间点LVEDP升高(P<0.05),LVSP和CF降低(P<0.05),灌注后15min冠脉流出液LDH活性增高(P<0.05),再灌注后45min后心肌MDA含量增高(P<0.05),梗死面积增大(P<0.05),电镜下心肌结构破坏明显;与无瑞芬太尼预处理组比较,两瑞芬太尼预处理组在灌注后各时间点LVEDP降低(P<0.05),LVSP以及CF升高(P<0.05),灌注后15min冠脉流出液LDH活性降低(P<0.05),再灌注后45min后心肌MDA含量降低(P<0.05),梗死面积较小(P<0.05),电镜下心肌结构破坏不明显;与RF1组比较,RF2组保护作用明显(P<0.05);结论:孕鼠离体心脏对缺血再灌输损伤的耐受下降,瑞芬太尼浓度依耐性地保护离体孕鼠全心缺血再灌注损伤。
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| 关 键 词： | 心肌缺血 心肌再灌注损伤/药物疗法 受体,阿片样,μ受体激动剂 模型,动物大 鼠 |
Protective effects of remifentanil preconditioning on myocardium against ischemia-reperfusion injury in pregnant rats |
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| Wu Mingguang Li Yuantao Huang Xiaolei et al. Protective effects of remifentanil preconditioning on myocardium against ischemia-reperfusion injury in pregnant rats[J]. Shaanxi Medical Journal, 2011, 40(1): 18-21 |
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| Authors: | Wu Mingguang Li Yuantao Huang Xiaolei et al |
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| Affiliation: | Wu Mingguang Li Yuantao Huang Xiaolei et al Department of Anesthesiology,Shenzhen Maternity and Child Healthcare Hospital of Southern Medical University(Shenzhen 518028) |
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| Abstract: | Objective :To observe the tolerance of the isolated hearts of pregnant mice on ischemia indoctrination injury and the protective effects of different concentrtion remifentanil pretreatment against pregnant rats myocardial ischemia reperfusion injury.Methods:40 male SD rats were randomly divided into 4 groups,10 non-pregnant mice were selected as control group.20 days after the pregnancy,30 pregnant rats were randomly divided into 3 groups:no remifentanil preconditioning group(RF0 group);low concentration re... |
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| Keywords: | Myocardial ischemia Myocardial reperfusion injury /drug therapy Receptor μ receptor agonist Model animal Rats |
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