| 人黑色素瘤A375细胞中B7-H4表达的实验研究 |
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| 引用本文： | 赵双,王万卷.人黑色素瘤A375细胞中B7-H4表达的实验研究[J].陕西医学杂志,2011,40(2):144-145,174. |
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| 作者姓名： | 赵双 王万卷 |
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| 作者单位： | 西安交通大学医学院第二附属医院皮肤科,西安,710004 |
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| 摘 要： | 目的:探讨B7-H4在人黑色素瘤A375细胞中表达及临床意义。方法:体外培养人黑色素瘤A375细胞,倒置显微镜观察细胞形态;采用RT-PCR和Western blot检测B7-H4 mRNA的转录水平及蛋白的表达水平。结果:B7-H4 mRNA和蛋白在A375细胞中表达均增高。结论:B7-H4在人黑色素瘤A375细胞中表达升高,可为黑色素瘤的诊断和治疗提供参考。
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| 关 键 词： | 黑色素瘤 实验性 细胞 培养的 逆转录聚合酶链反应 免疫印迹法 |
Experimental study on expression of B7-H4 in human melanoma A375 cells |
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| Zhao Shuang,Wang Wanjuan.Experimental study on expression of B7-H4 in human melanoma A375 cells[J].Shaanxi Medical Journal,2011,40(2):144-145,174. |
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| Authors: | Zhao Shuang Wang Wanjuan |
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| Institution: | Department of Dermatology,Second Hospital of Xi'an Jiaotong University(Xi'an 710004) Zhao Shuang Wang Wanjuan |
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| Abstract: | Objective:To study the expression and clinical significance of B7-H4 in human melanoma A375 cells.Methods:human melanoma A375 cells were cultured,the morphological alterations of A375 cells were observed by inverse microscopy.The mRNA and protein expression levels of B7-H4 were detected by RT-PCR and Western blot.Results: The expression of the mRNA and protein of B7-H4 increased in Human Melanoma A375 Cells.Conclusion:The expression of B7-H4 increased in human melanoma A375 cells,which can provide a referen... |
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