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| 蝶酸对天然蓖麻毒素及重组蓖麻毒素A链蛋白的拮抗作用研究 | |
| 引用本文： | 曹曦元,赵青,黎燕,沈倍奋,王玉霞,冯健男,彭晖. 蝶酸对天然蓖麻毒素及重组蓖麻毒素A链蛋白的拮抗作用研究[J]. 军事医学科学院院刊, 2010, 34(1): 12-15 |
| 作者姓名： | 曹曦元 赵青 黎燕 沈倍奋 王玉霞 冯健男 彭晖 |
| 作者单位： | 1. 军事医学科学院基础医学研究所,北京,100850 2. 天津大学药物科学与技术学院,天津,300072 3. 军事医学科学院毒物药物研究所,北京,100850 |
| 基金项目： | 国家“863”计划重大项目(2006AA02A254) |
| 摘 要： | 目的研究蝶酸（pterinacid,PTA）对天然蓖麻毒素（ricin）及重组蓖麻毒素A链蛋白（rRTA）的拮抗作用。方法用无细胞体系中荧光素酶合成抑制实验和体外细胞毒性实验评价PTA对全分子ricin和rRTA的拮抗效果。结果PTA在两种体系中均显示出了对ricin和rRTA的拮抗作用,且呈剂量依赖性。结论利用PTA作为小分子探针,建立了针对ricin/RTA小分子抑制剂的体外生物学评价系统,为以后用于拮抗ricin/RTA的全新小分子化合物筛选奠定了基础。 |
| 关 键 词： | 蝶酸 蓖麻毒素 重组蓖麻毒素A链蛋白 无细胞体系 抑制剂 |
Inhibitory effect of pterin acid against ricin and recombinant ricin A chain |
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| CAO Xi-yuan,ZHAO Qing,LI Yan,SHEN Bei-fen,WANG Yu-xia,FENG Jian-nan,PENG Hui. Inhibitory effect of pterin acid against ricin and recombinant ricin A chain[J]. Bulletin of the Academy of Military Medical Sciences, 2010, 34(1): 12-15 | |
| Authors: | CAO Xi-yuan ZHAO Qing LI Yan SHEN Bei-fen WANG Yu-xia FENG Jian-nan PENG Hui |
| Affiliation: | 1.Institute of Basic Medical Sciences/a>;Academy of Military Medical Sciences/a>;Beijing 100850/a>;China/a>;2.School of Pharmaceuticals and Technology/a>;Tianjin University/a>;Tianjin 300072/a>;3.Institute of Pharmacology and Toxicology/a>;China |
| Abstract: | Objective To study the inhibitory effect of pterin acid (PTA) against ricin and recombinant ricin A chain protein.Methods Luciferase protein synthesis inhibition assay in a cell-free system and in vitro cytotoxicity experiments were performed to assess the biological activity of ricin and rRTA treated with PTA.Results The result showed that PTA could significantly inhibit the activity of ricin and rRTA in a dose-dependent manner.Conclusion PTA might be used as a small molecular probe to develop an evaluatin... |
| Keywords: | pterin acid ricin recombinant ricin A chain protein cell-free system inhibitor |
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