中药HPLC指纹图谱相似性研究的探讨

目的探讨中药HPLC指纹图谱相似性评价中药质量的几个相关问题。方法建立中药HPLC指纹图谱相似性方法 ,包括色谱条件建立 ,系统适用性试验 ,稳定性考察 ,精密度试验 ,特征峰提取 ,对照样品的选择 ,共有模式的建立 ,及欧氏距离、相关系数、夹角余弦、指数相似系数、相似性比为测度的相似度。结果欧氏距离为测度的相似度侧重于特征变量值上大小差异的样品间亲疏程度相似性的定量信息描述 ,而其余 4种测度的相似度 ,在样品鉴别真伪上有优势 ,它们是反映特征变量变化模式上的样品间亲疏程度相似性的描述。这些相似度的计算均可在Excel软件上实施。结论中药HPLC指纹图谱相似性可以用来评价中药质量

Approaching the study on the similarity analysis of HPLC fingerprints spectra for traditional Chinese medicines

Objective To discuss the problem on the similarity analysis of HPLC fingerprints spectra for traditional Chinese medicines (TCM).Method The method was developed by the similarity analysis of HPLC fingerprints spectra which includes the establishment of chromatographic conditions, the test of system suitability, the check of stability, the test of precision, the extraction of character peak, the selection of reference, the establishment of common patterns,and the similarity by euclidean distance, Pearson correlation, cosine, exponent dispersion and similarity ratio.Result The similarity by the euclidean distance describes the information quantificationally about the characteristic variable sizes of samples.The similarity by the rest reflects the variety patterns of samples that have the advantage of identifying genuine and fake samples.The count of these similarities can be performed on the Excel software.Conclusion The quality of TCM was estimated by the similarity analyses of the HPLC fingerprints spectra.