瘢痕疙瘩成纤维细胞胶原合成的实验研究

目的 探讨瘢痕疙瘩中胶原过度聚集的原因.方法 从正常皮肤与活跃增生瘢痕疙瘩标本分别培养真皮成纤维细胞,采用³H-脯氨酸掺入法分别检测单层培养及胶原凝胶三维培养体系中成纤维细胞的胶原合成量.用斑点杂交法检测单层培养细胞的人前.α1(Ⅰ)型胶原mRNA水平.结果 瘢痕疙瘩成纤维细胞Ⅰ型胶原mRNA水平升高,其胶原合成量也显著高于正常真皮成纤维细胞(P<0.01).结论 在增生活跃瘢痕疙瘩中,成纤维细胞胶原合成功能处于活化状态,胶原合成增加是导致胶原过度积聚的重要原因.

Experimental Study on Collagen Synthesis in Cultured Keloid Fibroblasts

Objective To investigate the cause of excessive collagen accumulation in keloid tissue. Methods Human dermal fibroblasts were cultured from keloid and normaI skin.Collagen production by fi-broblasts in monolayer culture and in collagen gel was assessed by 3H-roline incorporation.Type I procollagen mRNA level of fibroblasts in monolayer culture was determined by dot blot hybridization using human proα(I)collagen-specific cDNA probe.Results The collagen synthesis significantly increased in keloid fibroblast lines (P<0.01), acompanied by a rise in type I procollagen-specific.mRNA levels in comparison with normal dermal fibroblast lines.Conclusion These findings suggest that the fihroblasts are activated in collagen syn-thesis in active keloid.The enhanced collagen synthesis by fibroblasts might contribute to excessive collagn ac-cumulation in keloid.