Intron-based genomic editing: a highly efficient method for generating knockin zebrafish |
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| Authors: | Jia Li Baibing Zhang Jiwen Bu Jiulin Du |
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| Affiliation: | 1. Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. School of Life Science and Technology, ShanghaiTech University, Shanghai 200031, China;3. University of Chinese Academy of Sciences, Shanghai 200031, China |
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| Abstract: | ![]()
The TALEN and CRISPR/Cas9 nuclease systems have been extensively utilized in genomic engineering of model organisms. In zebrafish, the nuclease systems have been successfully applied in generating loss-of–function knockout lines. However, genome-specific knockin techniques in zebrafish are still at the beginning. In this perspective, we briefly summarize the recent progresses on knockin approaches in zebrafish with a special focus on the newly developed intron-based knockin method. |
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| Keywords: | knockin CRISPR/Cas9 zebrafish |
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