SPARCL1 suppresses metastasis in prostate cancer |
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| Authors: | Yuzhu Xiang Qingchao Qiu Ming Jiang Renjie Jin Brian D. Lehmann Douglas W. Strand Bojana Jovanovic David J. DeGraff Yi Zheng Dina A. Yousif Christine Q. Simmons Thomas C. Case Jia Yi Justin M. Cates John Virostko Xiusheng He Xunbo Jin Simon W. Hayward Yajun Yi |
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| Affiliation: | 1. Department of Medicine, Vanderbilt University, Nashville, TN 37232-0275, USA;2. Minimally Invasive Urology Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;3. Vanderbilt Prostate Cancer Center and Department of Urologic Surgery, Vanderbilt University, Nashville, TN 37232-0275, USA;4. Institute for Integrative Genomics, Vanderbilt University, Nashville, TN 37232-0275, USA;5. Department of Biochemistry, Vanderbilt University, Nashville, TN 37232-0275, USA;6. Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232-0275, USA;7. Department of Pathology, Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232-0275, USA;8. Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN 37232-0275, USA;9. Cancer Research Institute and Human Morphology Center, University of South China, Hengyang 421001, China;10. Laboratory of Nuclear Receptors and Cancer Research, Center for Medical Research, Nantong University Medical School, Nantong, China |
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| Abstract: | ![]()
PurposeMetastasis, the main cause of death from cancer, remains poorly understood at the molecular level.Experimental designBased on a pattern of reduced expression in human prostate cancer tissues and tumor cell lines, a candidate suppressor gene (SPARCL1) was identified. We used in vitro approaches to determine whether overexpression of SPARCL1 affects cell growth, migration, and invasiveness. We then employed xenograft mouse models to analyze the impact of SPARCL1 on prostate cancer cell growth and metastasis in vivo.ResultsSPARCL1 expression did not inhibit tumor cell proliferation in vitro. By contrast, SPARCL1 did suppress tumor cell migration and invasiveness in vitro and tumor metastatic growth in vivo, conferring improved survival in xenograft mouse models.ConclusionsWe present the first in vivo data suggesting that SPARCL1 suppresses metastasis of prostate cancer. |
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| Keywords: | Prostate cancer Gene expression signature Meta-analysis Metastasis CaP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" cancer of the prostate gland H.E." },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" hematoxylin and eosin IC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" Intracardiac IHC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" Immunohistochemistry IVIS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" in vivo" },{" #name" :" __text__" ," _" :" Imaging System OX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" orthotopic xenografting PC3-Luc" },{" #name" :" keyword" ," $" :{" id" :" kwrd0100" }," $$" :[{" #name" :" text" ," _" :" the bioluminescent human prostate carcinoma cell line PC3-luc/EV" },{" #name" :" keyword" ," $" :{" id" :" kwrd0110" }," $$" :[{" #name" :" text" ," _" :" GFP-positive PC3-Luc cells expressing empty control vector PC3-luc/SPARCL1" },{" #name" :" keyword" ," $" :{" id" :" kwrd0120" }," $$" :[{" #name" :" text" ," _" :" GFP-positive PC3-Luc cells overexpressing SPARCL1 SCID" },{" #name" :" keyword" ," $" :{" id" :" kwrd0130" }," $$" :[{" #name" :" text" ," _" :" Severe combined immunodeficient SPARCL1" },{" #name" :" keyword" ," $" :{" id" :" kwrd0140" }," $$" :[{" #name" :" text" ," _" :" secreted protein acidic and rich in cysteine-like 1 |
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