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Optimization and validation of an 8-color intracellular cytokine staining (ICS) assay to quantify antigen-specific T cells induced by vaccination |
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| Authors: | Horton Helen Thomas Evan P Stucky Jason A Frank Ian Moodie Zoe Huang Yunda Chiu Ya-Lin McElrath M Juliana De Rosa Stephen C |
| Affiliation: | Program in Infectious Diseases, Fred Hutchinson Cancer Research Center (FHCRC), Seattle, WA 98109, USA. |
| Abstract: | |
| Keywords: | Intracellular Cytokine Staining (ICS) peripheral blood mononuclear cells (PBMC) HIV Vaccine Trials Network (HVTN) Cytomegalovirus (CMV) Human Immunodeficiency Virus (HIV-1) Staphylococcal enterotoxin B (SEB) potential T cell epitope (PTE) interferon-γ (IFN-γ) interleukin-2 (IL-2) tumor necrosis factor-α (TNF-α) interleukin-4 (IL-4). |
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