中药对小鼠氢化考的松模型小肠浆细胞IgG及腹膜肥大细胞的影响

本文以补肾中药对小白鼠氢考模型进行实验性治疗,应用 PAP 免疫组化法及吖啶橙活体染色法分别研究小肠 IgG 及腹膜肥大细胞的变化。初步发现补肾中药能使受氢考影响而下降的小肠 IgG 及腹膜肥大细胞量上升。提示补肾中药可能增强骨髓机能,使免疫活性细胞及肥大细胞增生,提高机体的免疫防御机能,从而对虚证可能有一定的调整作用。

The Effect of Chinese Medicine on IgG-Containing Plasma Cells and Peritoneal Mast Cells of Hydrocorticoid Mice

148 normal male mice,each weighing 23-29g,were selected for this experiment.The model was induced in some mice by hydrocortisone acetate(the dosage was 1mg per animal per day). Some were treated with 0.5ml of Chinese medicines(i.e.,Radix Aconiti Praeparata,Cortex Cinnamomi,Herba Epimedii,Radix Rehmanniae praeparata,Cornu Cervi and Radix Codonopsis Pilosulae,the dosage was altogether 0.25g)at the same time.The experiment lasted 7 days. After the last dose the animals were killed.The middle segment of the small intestine was stained by PAP immunohistochemical method to observe the IgG-containing plasma cells.The peritoneal mast cells were studied by cell counts and acridine orange vital staining method which was observed by a fluorescent microscope. Changes of IgG-containing plasma cells:Brown-black granules are present in the plasma cell cytoplasm.There were moderate numbers of IgG-containing plasma cells in the lamina propria of the small intestine of the controls.These cells decreased in the mice treated with hydrocortisone but increased markedly in those treated with Chinese medicines. Changes of mast cells:The mast cells were filled with bright orange or brick-red granules. The total number of mast cells of hydrocorticoid-treated mice decreased markedly.Its small- type cells decreased while the large ones increased.The total number of mast cells of the mice treated with Chinese medicines increased markedly.Its small-type cells increased and the large ones decreased. The B-lymphocyte originates in the pluripotential stem cells of bone marrow.The mast cell probably originates in the cell of thymus and bone marrow.The Chinese medicines mainly nourish the"kidney"and warm the"kidney"Yang in this experiment.These Chinese medicines probably improve the function of the bone marrow which produces immune activated cells and mast cells.These cells probably may regulate and decrease the svndrome of deficiency to some degree.