胃癌组织中HIF-1α,CD68及CD206在胃癌及癌旁组织中的表达及临床意义

目的 分析低氧诱导分子-1α（HIF-1α）及肿瘤相关巨噬细胞（TAM）相关抗原CD68、CD206在胃癌及癌旁组织中的表达情况。方法 利用免疫组化技术检测43例胃癌和癌旁组织中HIF-1α、CD68、CD206的表达状态,计算三种蛋白表达的阳性率及阳性细胞数,揭示其与临床因素的相关性。结果 HIF-1α、CD68、CD206的阳性表达率分别为58.1%、69.8%、51.2%,均高于癌旁组织（P<0.05）。胃癌、癌旁组织中每个视野下CD68⁺细胞数分别为（39.7±7.6）、（8.5±2.8）个;CD206⁺阳性细胞数分别为（32.0±9.2）、（3.4±1.8）个;HIF-1α⁺细胞数（22.9±5.6）、（2.1±1.2）个;组间比较差异均有统计学意义（P<0.01）。胃癌组织中CD206⁺细胞数与HIF-1α⁺细胞数表达呈正相关（P<0.01,R²=0.641）。三种蛋白的表达与胃癌病理分期、淋巴结转移明显相关（P<0.05）。结论 胃癌组织中CD68、CD206、HIF-1α表达率及阳性细胞数明显增加,且CD206与HIF-1α表达呈正相关。胃癌缺氧区域对M2型巨噬细胞有趋化作用,促进胃癌发生发展。

Expression of HIF-1,CD68 and CD206 in gastric cancer and its clinical significance

Objective To analyze the expression of hypoxic-induced molecule-1α(HIF-1α)and tumor-associated macrophages(TAM)related antigens CD68 and CD206 in gastric cancer and adjacent tissues.Methods Immunohistochemistry was used to detect the expression status of HIF-1α,CD68 and CD206 in 43 cases of gastric cancer and adjacent tissues,and the positive rate and number of positive cells of the three proteins were calculated to reveal their correlation with clinical factors.Results The positive expression rates of HIF-1,CD68 and CD206 were 58.1%,69.8%and 51.2%,respectively,which were higher than that of adjacent tissues(P<0.05).In gastric cancer and adjacent tissues,the number of CD68+cells in each field was(39.7±7.6)and(8.5±2.8),respectively;the number of CD206+positive cells was(32.0±9.2)and(3.4±1.8),respectively;the number of HIF-1α+cells was(22.9±5.6)and(2.1±1.2),respectively;the differences between groups were statistically significant(P<0.01).The number of CD206+cells in gastric cancer tissues was positively correlated with the expression of HIF-1α+cells(P<0.01,R2=0.641).The expression of the three proteins was related to pathological stage and lymph node metastasis(P<0.05).Conclusion The expression rates and the number of positive cells of CD68,CD206 and HIF-1αin gastric cancer tissues were significantly increased,and CD206 was positively correlated with the expression of HIF-1α.The hypoxia area of gastric cancer has chemotaxis effect on M2 macrophages and promotes the occurrence and development of gastric cancer.