丙烯酸-2-乙基己酯的遗传毒性研究

目的： 检测丙烯酸-2-乙基己酯的遗传毒性，为其安全性评价提供资料。方法：采用鼠伤寒沙门菌回复突变试验 (Ames试验)，小鼠骨髓嗜多染红细胞微核试验和小鼠淋巴瘤细胞TK基因突变试验检测丙烯酸-2-乙基己酯的遗传毒性。Ames试验设每皿0.08、0.4、2.0、10.0和50.0 μL剂量组，另设空白、溶剂、阳性对照；微核试验设350、700和1 400 mg/kg剂量组，另设溶剂、阳性对照；TK基因突变试验设12.5、25、50和100 μmol/L剂量组，另设溶剂、阳性对照。 结果：Ames试验中，加或不加S9的情况下，丙烯酸-2-乙基己酯对TA97、TA98、TA100和TA102菌株均显示致突变作用。微核试验中，雌雄鼠微核率均呈现剂量-反应关系；雄鼠高剂量组微核率与对照组比较差异具有统计学意义(P<0.05)；雌鼠各剂量组微核率与溶剂对照组比较差异无统计学意义(P>0.05)。TK基因突变试验中高剂量组突变频率与对照组比较，差异具统计学意义(P<0.05)。结论：在本实验条件下，Ames试验和TK基因突变试验均显示阳性结果，微核试验显示可疑阳性。丙烯酸-2-乙基己酯遗传毒性需结合其他检测系统综合评定。

The study of mutagenicity of 2-ethylhexyl acr ylate

OBJECTIVE：To add data about mutagenicity of 2-ethylhexyl acrylate（2-EHA）,so as to assess its safety. METHODS：Three genotoxicity tests,bacterial reverse mutation assay （Ames test）,micronucleus test of bone morrow cells and TK gene mutation assay with cell line L5178Y were performed in this study. Doses were designed at 0.08,0.4,2.0,10.0,50.0 μL/palte of Ames test,at 350,700,1 400 mg/kg of micronucleus test and at 12.5,25,50,100 μmol/L of TK gene mutation assay. RESULTS：In Ames test,2-EHA showed mutagenicity in Salmonella typhimurium TA97,TA98,TA100 and TA102 strains with or without S9. The micronucleus rate of the highest dose group in male mice （1 400 mg/kg） was significant increased compared with control group,while it was slightly increased in female mice. In TK gene mutation assay,the mutation frequency in two highest dose groups （50 and 100μmol/L） were significantly higher than that in control group. CONCLUSION：Under present experiental conditions,2-EHA showed genotoxicity in Ames test and TK gene mutation assay,micronucleus test were probably positive for 2-EHA. Genotoxicity of 2-EHA should be considered comprehensively by other mutagenicity tests.