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酶联免疫吸附试验监测梅毒的应用评价
引用本文:银燕,王丽萍. 酶联免疫吸附试验监测梅毒的应用评价[J]. 中国卫生检验杂志, 2008, 18(2): 308-309
作者姓名:银燕  王丽萍
作者单位:内蒙古消防总队医院,呼和浩特,010050
摘    要:
目的:评价酶联免疫吸附试验(ELISA)对梅毒病人诊断的敏感性和特异性。方法:使用目前常用的梅毒密螺旋体感染诊断酶联免疫吸附试验(ELISA)对3718例2006-7-1至2007-7-1门诊及住院手术输血病人进行检测。若结果阳性再做梅毒简介血凝试验(TPHA)。结果:ELISA共检阳性37例,其中30例经临床诊断为阳性。结论:该方法灵敏度高,操作方便。

关 键 词:酶联免疫吸附试验  梅毒间接血凝试验  敏感性  特异性  酶联免疫吸附试验  试验监测  梅毒密螺旋体  应用评价  syphilis  supervision  assay  immunosorbent  操作  灵敏度  临床诊断  TPHA  血凝试验  阳性  结果  检测  输血  手术  住院  感染诊断
文章编号:1004-8685(2008)02-0308-02
修稿时间:2007-09-24

Evaluation of enzyme-linked immunosorbent assay for supervision of syphilis
Yin Yan,Wang Li-ping. Evaluation of enzyme-linked immunosorbent assay for supervision of syphilis[J]. Chinses Journal of Health Laboratory Technology, 2008, 18(2): 308-309
Authors:Yin Yan  Wang Li-ping
Abstract:
Objective:To evaluate the sensitivity and specificity of ELISA in diagnosis of syphilis.Methods:The performance of an enzyme-linked immunosorbent assay(ELISA) technique for detection of treponema for the laboratory diagnosis of syphilis was evaluated in 3718 cases of outpatient and transfusion patient operated in hospital during 2006 to 2007.Patients with positive result were detected by treponema pallidum hemagglutination assay.Results:A total of 37 cases were positively examined by ELISA.Thirty of these 37 patients were positively diagnosed by clinical examination.Conclusion:ELISAs are highly sensitive and easily manipulated.
Keywords:Enzyme-linked immunosorbent assay  Treponema pallidum hemagglutination assay  Sensitivity  Specificity
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