不同促透剂对人参皂苷Rg1体外透皮吸收的影响

目的通过小鼠体外透皮实验,探讨不同促透剂对人参皂苷Rg1体外透皮吸收的影响,确定最佳促透剂,为研制新型透皮吸收制剂奠定基础。方法采用改良Franz扩散池法,以离体小鼠皮肤为透皮屏障,以生理盐水为接受介质,加入不同促透剂制备药液样品,以人参皂苷Rg1为定量指标成分进行体外透皮吸收检测,考察人参皂苷Rg1累计透过量。结果无促透剂条件下,人参皂苷Rg1未能透过;单一促透剂中以7%丙二醇效果最好,7%氮酮效果次之,冰片促透作用较小;复合促透剂中,氮酮＋丙二醇组各浓度下累计透过量接近,氮酮＋冰片组以两者质量浓度各为2.5%效果较好。结论常用促透剂丙二醇、氮酮、冰片均可增加药物中人参皂苷Rg1的透皮吸收量,其中,7%丙二醇为最佳促透剂。

Effects of different penetration enhancers on transdermal absorption of ginsenoside Rg_1 in vitro

Objective To investigate the effects of different penetration enhancers on transdermal absorption of ginsenoside Rg1in vitro through skin-penetrating experiments in mice,and determine the best penetration enhancer for laying the foundation for researching new transdermal absorption preparations.Methods Using the modified Franz diffusion pool method,with mice skin as transdermal barrier,physiological saline as accept medium,adding different penetration enhancers to make samples,with ginsenoside Rg1as quantitative indexes for transdermal absorption in vitro tests,measured by high performance liquid chromatography（HPLC） method,inspects the accumulation of ginsenoside Rg1through the volume.Results Without penetration enhancers,ginsenoside Rg1had no accumulation; In single penetration enhancers,7%propylene glycol had the best effect,7% azone came second,borneol in aqueous solution had more lower effect; In compound penetration enhancers,the accumulation of azone ＋ propylene glycol groups was closing at any concentrations; Effect of azone＋ borneol group was better when both concentrations were at 2.5%.Conclusion Common penetration enhancers as azone,propylene glycol and borneol can increase the accumulation of ginsenoside Rg1,among them,7% propylene glycol was the best.