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Exosomes from human saliva as a source of microRNA biomarkers |
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| Authors: | A Michael SD Bajracharya PST Yuen H Zhou RA Star GG Illei I Alevizos |
| Affiliation: | Sjögren's Syndrome Clinic, Molecular Physiology &Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA;;Renal Diagnostics and Therapeutics Unit, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA |
| Abstract: | Objective: The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications. Design: Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays. Results: We successfully isolated exosomes from human saliva from healthy controls and a patient with Sjögren's syndrome. microRNAs extracted from the exosomal fraction were sufficient for quantitative PCR and microarray profiling. Conclusions: The isolation of miRNAs from easily and non-invasively obtained salivary exosomes with subsequent characterization of the miRNA expression patterns is promising for the development of future biomarkers of the diagnosis and prognosis of various salivary gland pathologies. |
| Keywords: | salivary exosomes microRNA biomarkers |