乙酰唑胺对内毒素血症鼠胃黏膜水通道蛋白4表达的影响

目的探讨乙酰唑胺对内毒素血症鼠胃黏膜水通道蛋白4（AQP4）表达的影响及意义。方法健康SD大鼠30只，随机分成三组：对照组、内毒素血症组（EDT组）和乙酰唑胺作用组（ATL组），每组10只，免疫组织化学法检测各组大鼠胃黏膜中AQP4的表达，并同时放免法测定其血清肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。结果EDT组AQP4蛋白表达及血清TNF-α、IL-1β水平较对照组明显增加（均P〈0．01）。乙酰唑胺作用后，A1L组的胃黏膜上皮水通道AQP4蛋白表达及血清TNF—α、IL-1β水平均明显低于EDT组（均P〈0．01）。结论AQP4表达的增加和血清TNF-α、IL-1β水平增高参与了内毒素血症大鼠急性胃黏膜组织的损害。乙酰唑胺可下调胃黏膜上皮水通道蛋白AQP4的表达并降低血清TNF—α，IL-1β水平，减轻内毒素对胃黏膜上皮组织造成的损害，保护胃黏膜组织。

Effects of Acetazolamide on the expression of aquaporin-4 of gastric mucosal in rats with endotoxemia

Objective To explore the effects and significance of acetazolamide on the expression of aquaporin-4(AQP4) of gastric mucosal in rats with endotoxemia. Methods 30 SD rats were randomly divided into 3 groups: Sham operated control group( Control group) ,endotoxemia group( EDT group) and acetazolamide group( ATL group).The immunohistochemistry was used to assess the expression of the AQP4 in the gastric mucosal tissues. The serum tumor necrosis factor-α(TNF-α) and interleukin-1 β(IL-1β) levels in each group were measured by radioimmunoassay. Results The expression of AQP4 and the levels of serum TNF-αt and IL-1 ββ in EDT group were higher than those in control group( P ＜ 0.01 ). After the ALT treatment, the expression of AQP4 and the levels of serum TNF-α and IL1 β in ALT group were lower than those in EDT group( all P ＜0.01 ) and the expression of AQP4 in the gastric mucosal tissues in ALT group was also lower than those in EDT group( P ＜0.01 ). Conclusion The raising of AQP4 expression and increasing of serum TNF-α, IL-1β take part in the progress of gastric mucosal injury in rats with endotoxemia. ALT could protect the gastric mucosal tissues by lowering the AQP4 expression and reducing the levels of serum TNF-α and IL-l β.