| 放疗对人肝癌细胞株Hep-G2中Bax和Bcl-2表达的影响 |
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| 引用本文： | 张力,何瑞龙,崔丽华,张立广,龚明玉.放疗对人肝癌细胞株Hep-G2中Bax和Bcl-2表达的影响[J].承德医学院学报,2007,24(2):121-123. |
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| 作者姓名： | 张力 何瑞龙 崔丽华 张立广 龚明玉 |
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| 作者单位： | 承德医学院附属医院放疗科,河北,承德,067000;承德医学院 |
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| 摘 要： | 目的:探讨放疗对人肝癌细胞株Hep-G2中Bax和Bcl-2蛋白表达水平的影响.方法:6MV-X射线照射细胞,采用免疫组织化学染色观察人肝癌细胞株中Bax和Bcl-2的表达.结果:Bax和Bcl-2的表达在照射时间和照射剂量之间存在交互作用(F=1.733,P=0.042;F=1.700,P=0.048).4Gy照射剂量作用细胞48h后,Hep-G2细胞中Bax的表达达高峰、Bcl-2的表达至低谷.结论:放射线可能通过促进Bax表达和抑制Bcl-2表达诱导肝癌细胞凋亡.
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| 关 键 词： | X线 人肝癌细胞株Hep-G2 细胞凋亡 bcl-2 bax |
| 文章编号： | 1004-6879（2007）02-0121-03 |
| 收稿时间： | 2006-12-25 |
| 修稿时间： | 2006-12-25 |
INFLUENCE OF ACTINOTHERAPHY ON EXPRESSION OF BCL-2 AND BAX IN HUMAN HEPATOMA CELL LINE HEP-G2 |
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| ZHANG Li, HE Rui-long, CUI Li-hua, et al.INFLUENCE OF ACTINOTHERAPHY ON EXPRESSION OF BCL-2 AND BAX IN HUMAN HEPATOMA CELL LINE HEP-G2[J].Journal of Chengde Medical College,2007,24(2):121-123. |
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| Authors: | ZHANG Li HE Rui-long CUI Li-hua |
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| Institution: | Department of Radiation Oncology, Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000,China |
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| Abstract: | Objective:To explore the influence of actinotheraphy on expression of bcl-2 and bax in human hepatoma cell line Hep-G2. MethodsrHuman hepatoma cell line Hep-G2 were exposured under 6-MV X-ray radiation and the expression of Bax and Bcl-2 protein were detected with immunohitochemistry staining. Results: The expression of Bax and Bcl-2 protein have interaction in time and radiation dose (F= 1.733, P=0.042; F=1. 700,P=0.048). 48 hours after the cell line Hep-G2 were exposured under 4Gy radiation, the expression of Bax increased to the peak and Bcl-2 decreased to the minimal level.Condusions: Irradiation could induce apoptosis of human hepatoma cell line Hep-G2 by up-regulating bax expression and down-regulating bcl-2 expression. |
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| Keywords: | bcl-2 bax |
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