胃癌中uPA、PAI-1表达及其与血管生成的关系

目的 观察胃癌组织中uPA、PAI-1mRNA及蛋白的表达，并探讨它们与肿瘤分化、血管生成及临床病理因素之间的关系。方法 用原位杂交及免疫组化S-P法检测110例胃癌组织中uPA、PAI-1的表达，根据CD34阳性的血管内皮细胞计数肿瘤组织微血管密度（MVD）。结果 （1）胃癌组织中uPA mRNA和蛋白、PAI-1 mRNA和蛋白阳性表达定位于胞质；uPA的表达随分化程度的降低有逐渐升高的趋势，PAI-1的表达随分化程度的降低有逐渐降低的趋势。（2）110例uPA mRNA及蛋白表达阳性组MVD值显著高于阴性组，差异均具有显著性（P值均＜0．05）。（3）uPA mRNA及蛋白的表达与临床分期呈正相关（P＜0．05），PAI-1的表达与临床分期和淋巴结转移无相关性。（4）uPA mRNA／蛋白与PAI-1 mRNA／蛋白的表达无相关性。结论uPA与促进胃癌的血管生成密切相关，阻断uPA的分泌和作用途径有望对胃癌浸润转移起抑制作用；胃癌组织中PAI-1可能担当重要的调节剂或者是肿瘤细胞防止自身降解的保护剂而不是这个系统的单纯抑制剂。

Correlation of the expression of uPA and PAI-1 with angiogenesis in human gastric cancer

Purpose To investigate the expression of urokinase-type plasminogen(uPA),its inhibitor-1(PAI-1) mRNA and protein in human gastric cancer and its relationship to tumor differentiation,angiogenesis,and clinical pathologic factors.Methods In situ hybridization(ISH) for uPA and PAI-1 mRNA was performed in 110 cases of human gastric cancer prepared within 2 blocks of tissue microarray(TMA).Immunohistochemical staining(S-P method) for uPA and PAI-1 protein and CD34 was performed in 110 cases of human gastric cancer.Results uPA,PAI-1 mRNA and protein were located in the cytoplasm of gastric cancer cells;they were all correlated with the histologic differentiation.The MVD in uPA positive group was significantly higher than those in negative group(P<0.05);uPA positive expression and MVD had a positive correlation with the clinical stages(P<0.05).The expression of PAI-1 had no correlation with the clinical stages and lymph node metastasis.Conclusions uPA is significantly correlated with angiogenesis,invasion and metastasis of gastric cancer;PAI-1 may be an inhibitor factor involved in the growth and invasion of gastric cancer.