白念珠菌厚膜孢子形态发生过程的扫描电镜观察

目的 用扫描电镜观察白念珠菌厚膜孢子在液体培养基中形态发生过程。方法 用改良液体培养基培养白念珠菌厚膜孢子，在不同培养时间收获离心，制备扫描电镜样品，然后观察不同培养时相厚膜孢子及假菌丝的形态。结果 发现培养３小时芽生孢子发芽。５小时芽体延长；７小时次极芽体伸长，假菌丝形成，顶端孢子开始增大；９小时假菌丝延长，厚膜孢子进一步成熟；１６小时厚膜孢子成熟；２４小时见多数厚膜孢子和假菌。结论 用该方法能

OBSERVATION ON THE MORPHOGENESIS OF CHLAMYDOSPORES OF CANDID ALBICANS BY USE OF SCANNING ELECTRON MICROSCOPY

The morphogenesis of chlamydospores of Candida albicans was observed by use of scanning electron microscopy. Results showed that the yeast cells germinated after 3h of cultivation,the budding-cells elongated after 5h, and the pseudohyphae formed and the mother cells began chlamydosporulation at about 7h of growth. Subsequently, pseudohyphal e1ements elongated by 9h;the characteristic chlamydospores formed at about 16h,and after 24h of cultivation, numbers of chamydospores - pseudohyphae and yeast - form elements can be found. We come to the conculsion that the process of morphogenesis for the chlamydospores of C. albicans can be observed clearly by scanning electron microscopy.