结核分枝杆菌部分耐药相关基因的突变特点

目的 分析结核分枝杆菌耐药相关基因的突变特点,评价DNA序列分析用于结核分枝杆菌快速耐药性检测的应用价值.方法 利用DNA序列分析检测结核分枝杆菌的katG、rpoB和gyrA基因片段.结果 86.81%(79/91)异烟肼耐药株存在katG基因的点突变和/或单碱基插入,最常见的突变形式为R463L,占76.92%(70/91).90.38%(94/104)的利福平耐药株在rpoB基因耐药决定区(RRDR)发生突变,突变类型有26种,涉及11种氨基酸,突变集中在密码子第507～533位.70.49%(86/122)氧氟沙星耐药株在gyrA基因耐药决定区(QRDR)发生突变,突变主要形式为A90V、D94Y、D94N、D94H及S91P.结论 耐药相关基因突变的检测,可作为临床快速检测结核分枝杆菌耐药性的方法之一.

Characterization of some gene mutations associated with drug-resistance in Mycobacteria tuberculosis

Objective To analyze the characterization of drug-resistance associated genes of Mycobacteria tuberculosis and evaluate its clinical significance.Method DNA sequencing were performed on the gene fragment of katG,rpoB and gyrA of Mycobacteria tuberculosis.Results 86.81%(79/91)INH-resistant clinical strains had point mutation and/or single-base insertion in katG.There are 26 types of mutation,which refer to 11 amino acids, the most common type was R463L.90.38%(94/104)RFP-resistant strains had gene mutations in rpoB,the most common mutations located in 507.533 codons.70.49%(86/122)OFLX-resistant strains had mutations in gyrA,the most common types were A90V,D94Y,D94N,D94H and S91P.Conclusions Mutations of drug-resistant associated genes is valuable to analyze the drag-resistant of Mycobacteria tuberculosis rapidly.