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| hCAP-18／LL-37全长肽真核表达产物对树突状细胞分化成熟的影响 | |
| 引用本文： | 袁红艳,许娜,王凤丽,常雅萍. hCAP-18／LL-37全长肽真核表达产物对树突状细胞分化成熟的影响[J]. 中国实验诊断学, 2010, 14(3): 346-348 |
| 作者姓名： | 袁红艳 许娜 王凤丽 常雅萍 |
| 作者单位： | 1. 吉林大学白求恩医学院免疫学教研室,吉林,长春130021 2. 吉林大学白求恩医学院免疫学教研室,吉林,长春130021;吉林医药学院,吉林,吉林市132013 |
| 基金项目： | 吉林省科学技术厅发展计划项目 |
| 摘 要： | 目的研究人源阳离子抗菌肽（hCAP）－18／LL-37全长肽真核表达产物对树突状细胞分化成熟的影响。方法将已构建的hCAP-18／LL-37全长肽真核表达载体pcDNA4／Myc－His-hCAP-18转染Hela细胞株，在转染后48h收获培养上清；人外周血单个核细胞分离，体外定向诱导分化成树突状细胞（Dc）；将转染后收获的培养上清与体外培养的Dc共同孵育，48h收获Dc，经流式细胞仪检测DC表面分子CD83、CD86、CIM0及mA．DR的表达。结果FCM结果显示：pcDNA4／Myc—His-hCAP-18转染Hela细胞培养上清与pcDNA4／Myc-His空载体转染细胞培养上清相比较，均可上调Dc表面分子CD86、CIMO及HLA—DR的表达。结论构建的hCAP-18／LL-37全长肽真核表达载体pcDNA4／Myc。His-hCAP-18转染Hela细胞株培养上清液均可上调Dc表面分子CD86、CIMO及HLA—DR的表达，促进Dc的分化成熟。 |
| 关 键 词： | 抗菌肽 hCAP-18／LL-37 真核表达 树突状细胞 |
The effects of eukaryotic expression products of the whole range peptide of hCAP-18/LL-37 on differentiation and maturation of DC |
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| YUAN Hong-yan,XU Na,,WANG Feng-li,et al.. The effects of eukaryotic expression products of the whole range peptide of hCAP-18/LL-37 on differentiation and maturation of DC[J]. Chinese Journal of Laboratory Diagnosis, 2010, 14(3): 346-348 | |
| Authors: | YUAN Hong-yan XU Na WANG Feng-li et al. |
| Affiliation: | YUAN Hong-yan1,XU Na1,2,WANG Feng-li1,et al.(1.Immunology Department of Bethune Medical College,Jilin University,Changchun 130021,China,2.Jilin Medical College) |
| Abstract: | Objective Objective To study The effects of eukaryotic expression products of the whole range peptide of hCAP-18/LL-37 on differentiation and maturation of DC.Methods pcDNA4/Myc-His-hCAP-18 for the whole peptide was transfected into Hela cell line.After the cells were cultured for 48 h the supernatant was collected.We prepared human peripheral blood monouclear cells and induce them into DC in vitro.Then we add the supernatant collected from the transfected cells and continued culturing the DC for 48 h.By FC... |
| Keywords: | hCAP-18/LL-37 |
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