| 人参皂苷Rh2对白血病HL-60细胞凋亡的诱导作用 |
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| 引用本文： | 李罗丝,罗志勇,文斌,刘水平,张阳德. 人参皂苷Rh2对白血病HL-60细胞凋亡的诱导作用[J]. 中国现代医学杂志, 2006, 16(21): 3215-3217 |
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| 作者姓名： | 李罗丝 罗志勇 文斌 刘水平 张阳德 |
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| 作者单位： | 1. 中南大学卫生部肝胆肠外科研究中心,湖南,长沙,410008
2. 中南大学湘雅医学院,分子生物学研究中心,湖南,长沙,410078 |
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| 摘 要： | 目的探讨人参皂苷Rh2对人白血病HL-60细胞增殖和凋亡的影响。方法应用MTT法检测人参皂苷Rh2对HL-60细胞增殖的影响，采用DNA的片段化实验，观察人参皂苷Rh2对HL-60细胞凋亡的影响。结果G—Rh2能明显抑制HL-60细胞增殖，呈浓度依赖性关系；人参皂苷Rh2能明显诱导HL-60细胞凋亡，呈浓度和时间依赖效应，DNA的琼脂糖凝胶电泳分析可见凋亡特征性的DNA梯状图谱。结论G—Rh2主要通过有效抑制HL-60细胞增殖并诱导其凋亡从而发挥其抗白血病效应。
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| 关 键 词： | 人参皂苷Rh2 HL-60细胞 增殖 细胞凋亡 |
| 文章编号： | 1005-8982（2006）21-3215-03 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
Effect of Ginsenoside Rh2 on the proliferation and apoptosis in HL-60 cells |
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| LI Luo-si,LUO Zhi-yong,WEN Bin,LIU Shui-ping,ZHANG Yang-de. Effect of Ginsenoside Rh2 on the proliferation and apoptosis in HL-60 cells[J]. China Journal of Modern Medicine, 2006, 16(21): 3215-3217 |
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| Authors: | LI Luo-si LUO Zhi-yong WEN Bin LIU Shui-ping ZHANG Yang-de |
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| Affiliation: | 1.National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Central South University, Changsha, Hunan 410008, P.R.China; 2. Molecular Biology Research Centre, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, P.R.China |
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| Abstract: | [Objective] To investigate effect of Ginsenoside Rh2 on the proliferation and apoptosis in HL-60 cells. [Method] Effects of Ginsenoside Rh2 on the HL-60 cells proliferation and apoptosis were determined by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrasodium bromide] and DNA fragmentation assays, respectively. [Results] Ginsenoside Rh2 can significantly inhibit the proliferation of HL-60 cells in the dose-dependent manner. In addition, apoptosis of HL-60 cells can be induced by Ginsenoside Rh2 in the dose- and time-dependent manners. Apoptosis-characteristic DNA ladders can be observed by DNA agarose gel electrophoresis. [Conclusions] Ginsenoside Rh2 plays an crucially important role in exerting its anti-leukemic effects via inhibition of proliferation and induction of apoptosis in HL-60 cells. |
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| Keywords: | Ginsenoside Rh2 HL-60 cells proliferation apeptosis |
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